SARS-COV-2 Spike RBD (N501Y, K417N, E484K) Protein (COV-VM1RM)
Artikelnummer:
KAB-COV-VM1RM
Hersteller Artikelnummer:
COV-VM1RM
Alternativnummer:
KAB-COV-VM1RM-100UG, KAB-COV-VM1RM-500UG
Hersteller:
KactusBio
Kategorie:
Proteine/Peptide
Spezies Reaktivität:
Virus
Alternative Synonym:
S protein RBD, S glycoprotein RBD, Spike protein RBD, sars-cov-2
Recombinant SARS-COV-2 Spike RBD (N501Y,K417N,E484K) Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Arg319-Phe541(N501Y,K417N,E484K).
Molekulargewicht:
The protein has a predicted MW of 26.2 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Bis-Tris PAGE result.
Tag:
C-His
UniProt:
QHD43416.1
Puffer:
Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Reinheit:
> 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Formulierung:
Lyophilized
Sequenz:
Arg319-Phe541(N501Y,K417N,E484K)
Target-Kategorie:
Spike RBD
SARS-COV-2 Spike RBD (N501Y,K417N,E484K) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Immobilized SARS-COV-2 Spike RBD (N501Y,K417N,E484K) , His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 9.9ng/ml determined by ELISA.
Loaded Human ACE2, hFc Tag on ProA-Biosensor can bind SARS-COV-2 Spike RBD (N501Y, K417N, E484K), His Tag with an affinity constant of 12.90 nM as determined in BLI assay (Gator Prime).
The purity of SARS-COV-2 Spike RBD (N501Y,K417N,E484K) is greater than 95% as determined by SEC-HPLC.
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