Human TGF-alpha Protein (TGF-HM201)

Produktübersicht

• Artikelname: Human TGF-alpha Protein (TGF-HM201)
• Artikelnummer: KAB-TGF-HM201
• Hersteller Artikelnummer: TGF-HM201
• Alternativnummer: KAB-TGF-HM201-100UG, KAB-TGF-HM201-500UG
• Hersteller: KactusBio
• Kategorie: Proteine/Peptide
• Spezies Reaktivität: Human
• Alternative Synonym: ETGF, TGF-alpha, TGF type 1, TGFA

Produktbeschreibung

Recombinant Human TGF-alpha Protein is expressed from HEK293 with hFc (IgG1) tag at the N-Terminus.It contains Val40-Ala89.

Produkteigenschaften

• Molekulargewicht: The protein has a predicted MW of 32.9 kDa. Due to glycosylation, the protein migrates to 38-45 kDa based on Bis-Tris PAGE result.
• Tag: N-hFc (IgG1)
• UniProt: P01135
• Puffer: Lyophilized from 0.22µm filtered solution in 50mM Tris,100mM NaCl (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Reinheit: > 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
• Formulierung: Lyophilized
• Sequenz: Val40-Ala89
• Target-Kategorie: TGF-alpha

Bilder

Human TGF-alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human TGF-alpha is greater than 95% as determined by SEC-HPLC.

Human EGFR, His Tag captured on CM5 Chip via anti-his antibody can bind Human TGF-alpha, hFc Tag with an affinity constant of 0.31 uM as determined in SPR assay (Biacore T200).

Immobilized Human TGF-alpha, hFc Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-TGF-alpha Antibody, hFc Tag with the EC50 of 6.0ng/ml determined by ELISA (QC Test).

Loaded Human TGF-alpha, hFc Tag on ProA-Biosensor can bind Human EGFR, His Tag with an affinity constant of 0.27 µM as determined in BLI assay (Gator Prime).