Suggested starting dilutions are as follows: Western blot: 1:500-1:3000, IHC-Paraffin: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0)], ICC/IF: 1:100-1:1000. Not yet tested in othe
Sample (30 ug of whole cell lysate) A: NT2D1 B: U87-MG 7.5% SDS PAGE CDYL antibody
IHC of paraffin-embedded BT474 xenograft, using CDYL antibody at 1:250 dilution.
Immunofluorescence of paraformaldehyde-fixed HeLa, using CDYL antibody at 1:500 dilution.
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