50 mM Sodium Phosphate, pH 7.5, 150 mM NaCl, 100 mM KCl, 0.04% Sodium Azide, 5% Glycerol, 0.2% BSA
Reinheit:
Protein A purified
Formulierung:
50 mM Sodium Phosphate, pH 7.5, 150 mM NaCl, 100 mM KCl, 0.04% Sodium Azide, 5% Glycerol, 0.2% BSA
Application Verdünnung:
ELISA, Flo
Anwendungsbeschreibung:
Ficoll prepared human peripheral blood mononuclear cells (PBMC) were stimulated overnight in culture with 5 ug/ml PHA. Five x 105 cultured human U-937 cells per tube were washed and pre incubated 5 minutes with 20 l of 300 g/ml human IgG (to reduce non
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