50 mM Sodium Phosphate, pH 7.5, 150 mM NaCl, 100 mM KCl, 0.04% Sodium Azide, 5% Glycerol, 0.2% BSA
Reinheit:
Protein A purified
Formulierung:
50 mM Sodium Phosphate, pH 7.5, 150 mM NaCl, 100 mM KCl, 0.04% Sodium Azide, 5% Glycerol, 0.2% BSA
Application Verdünnung:
Flo
Anwendungsbeschreibung:
Ficoll prepared human peripheral blood mononuclear cells (PBMC) were stimulated 5 days in culture with 5 ug/ml Phytohemagglutanin (PHA). Five x 105 cells per tube were pre incubated 10 minutes with 250 ug/ml human IgG (to block nonspecific binding) after
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