The VP40 protein is critical in viral assembly and interacts with viral ribonucleocapsid and host ESCRT proteins (VPS4, PDCD6IP/ALIX, NEDD4, TSG101). It promotes efficient budding and binds to the host E3 ubiquitin ligase SMURF2. VP40 Protein, Zaire ebolavirus (H269L) is the recombinant Virus-derived VP40 protein, expressed by E. coli , with tag free.