2-Ethylbutyric acid is a volatile fatty acid. 2-Ethylbutyric acid exhibits phytotoxicity. 2-Ethylbutyric acid is used as an internal standard in milk and dairy products and as an internal standard (IS) in the calibration of fecal VFA standards. 2-Ethylbutyric acid can be used in the research of embryonic development[1][2][3][4].