MD Cryo-EM Vitrification Screen

Produktübersicht

• Artikelname: MD Cryo-EM Vitrification Screen
• Artikelnummer: MDL-MD1-911
• Hersteller Artikelnummer: MD1-911
• Alternativnummer: MDL-MD1-911
• Hersteller: Molecular Dimension
• Kategorie: Kits/Assays

Produktbeschreibung

The MD Cryo-EM Vitrification Screen is a rationally designed additive screen of 19 different conditions aimed at improving vitrification outcomes in single-particle cryo-electron microscopy workflows.

Zusatzinformationen

The MD Cryo-EM Vitrification Screen (MD1-911) is a rationally designed additive screen of 19 different conditions aimed at improving vitrification outcomes in single-particle cryo-electron microscopy workflows. It systematically addresses the most common sample preparation bottlenecks, including air–water interface (AWI) adsorption, preferred orientation, aggregation, and uneven ice - by providing a diverse set of validated detergents and surfactants for buffer and additive screening.

Developed by Molecular Dimensions in collaboration with Anatrace, this screen brings together over 60 years of combined expertise in detergents, surfactants, and cryo-EM sample optimisation.

• Targets adsorption, orientation bias, particle distribution, and ice thickness
• Wide chemical space: detergents, amphipols, fluorinated surfactants
• Built on peer-reviewed literature and real-world cryo-EM successes
• Compatible with standard vitrification systems (Vitrobot, Chameleon, automatic plunge-freezers)

 

---

 

Features and Benefits

Comprehensive Additive Coverage

Designed with a sparse-matrix approach, MD1-911 spans multiple additive classes; non-ionic detergents, zwitterionic surfactants, amphipols, fluorinated detergents, providing breadth in cryo-EM screening and troubleshooting.

Research-Proven Conditions

Includes conditions cited in cryo-EM publications and validated across diverse challenging targets proven conditions that have improved particle behaviour on grids.

Improve Particle Distribution: Screen for conditions that promote even dispersion and reduce aggregation.
Lower Preferred Orientation: Use targeted surfactants and amphipols to overcome orientation bias.
Mitigate AWI Adsorption: Conditions designed to keep particles off the damaging air–water interface.
Boost Ice Quality: Identify additives that stabilise thin, uniform vitreous ice.

Workflow-Ready Format

Convenient 10× concentrated conditions ready to spike into protein samples.

 

---

 

Workflow

1. Thaw screen
2. Mix solutions directly with sample (e.g. 4.5 µL protein + 0.5 µL of 10× condition)
3. Prepare grids via standard vitrification
4. Assess particle behaviour, distribution, orientation, and ice

Bilder