Citrate buffer, Heat-Induced Epitope Retrieval (10x, Tween20), Red color

Produktübersicht

• Artikelname: Citrate buffer, Heat-Induced Epitope Retrieval (10x, Tween20), Red color
• Artikelnummer: MON-APP919R
• Hersteller Artikelnummer: MON-APP919R
• Alternativnummer: MON-APP919R
• Hersteller: Monosan
• Kategorie: Sonstiges
• Applikation: IHC

Produktbeschreibung

The Citrate buffer, is designed for use during heat-induced epitope retrieval (HIER) on formalinfixed paraffin-embedded tissue sections prior to antibody application. In immunohistochemistry (IHC), protein cross-links are formed during formalin or other aldehyde fixation. These cross-links mask the antigenic sites in tissue specimens and results in weak or false negative staining in immunohistochemical detection of proteins. The use of this citrate buffer in combination with heat ensures that the antigenicity of proteins modified, thus enhancing staining intensity of antibodies. For the recommended pre-treatment technique, please refer to the individual antibodys instructions for use. This buffer is a 10x concentrate solution and must be diluted 1:10 with deionized water before using. The final readyto-use solution should have a pH of 6.0 0.3. The buffer can be used for a maximum of three runs within five days after dilution. Also available in 250 ml, 500 ml en 1000 ml.