The 3CLglowLive Assay kit is well suited to experiments using the live SARS-CoV-2 virus. It is dim at baseline but fluoresces green in the presence of 3CL protease activity, an indicator of SARS-CoV-2 viral replication. The kit includes 2 BacMam viruses - one expressing the 3CLglowLive biosensor and the other a 3CLpro enzyme, which could be used to test the biosensor and optimize fluorescence detection settings.
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