This MAb recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. In Western blotting of cell lysates from 7 human breast cancer cell lines (ZR75-1, ZR75-30, MCF-7, MDAMB453, T47D, CAL51, 734B), the antibody labels a single band corresponding to p27Kip1. It functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. Reportedly, low p27 expression has been associated with unfavorable prognosis in renal cell carcinoma, colon carcinoma, breast carcinomas, non-small-cell lung carcinoma, hepatocellular carcinoma, multiple myeloma, and lymph node metastases in papillary carcinoma of the thyroid, as well as a more aggressive phenotype in carcinoma of the cervix.
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Anwendungsbeschreibung:
Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45
Flow Cytometry of human p27 on He
Western blot analysis of Raw lysate using p27 Mouse Monoclonal Antibody (SX53G8).
Western blot analysis of 3T3 lysate using p27 Mouse Monoclonal Antibody (SX53G8).
Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with p27 Mouse Monoclonal Antibody (SX53G8).
Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with p27 Mouse Monoclonal Antibody (SX53G8).
Flow Cytometry of human p27 on HeLa cells. Black: cells alone, Grey: Isotype Control, Green: AF488-labeled p27 Mouse Monoclonal Antibody (SX53G8).
Immunofluorescence Analysis of PFA-fixed MCF-7 cells labeling p27 with p27 Mouse Monoclonal Antibody (SX53G8) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is stained with Phalloidin-CF640
Flow Cytometric Analysis of PFA-fixed MCF-7 cells using p27 Mouse Monoclonal Antibody (SX53G8) followed by Goat anti- Mouse- IgG-CF488 (Blue), Isotype Control (Red).
SDS-PAGE Analysis of Purified p27 Mouse Monoclonal Antibody (SX53G8). Confirmation of purity and integrity.
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