Multiple isoelectric variants of calponin have been identified, however only two molecular weight isoforms exist, a 34kDa form and a 29kDa form. Expression of the 29kDa form, I-calponin, is primarily restricted to muscle of the urogenital tract, whereas the higher molecular weight variant has been demonstrated in vascular and visceral smooth muscle. In Western blotting, this MAb reacts with only the 34kDa form of calponin in extracts of human aortic medial smooth muscle and is unreactive with fibroblast extracts of cultivated human foreskin. Calponin is a calmodulin, F-actin and tropomyosin binding protein, which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle.
200ug/ml of Ab Purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Recombinant Monoclonal Antibody
Anwendungsbeschreibung:
Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min
SDS-PAGE Ana
Formalin-fixed, paraffin-embedded human Uterus stained with Calponin Rabbit Recombinant Monoclonal Antibody (CNN1/1408R).
Formalin-fixed, paraffin-embedded Rat Uterus stained with Calponin Rabbit Recombinant Monoclonal Antibody (CNN1/1408R).
SDS-PAGE Analysis of Purified Calponin Rabbit Recombinant Monoclonal Antibody (CNN1/1408R).
Flow Cytometric Analysis of PFA-fixed K562 cells using Calponin Rabbit Recombinant MAb(CNN1/1408R) followed by Goat anti-Rabbit- IgG-CF488 (Blue), Isotype Control (Red).
Immunofluorescence Analysis of Human K562 cells labeling CD71 with Calponin Rabbit Recombinant Monoclonal Antibody (CNN1/1408R)followed by Goat anti-rabbit IgG-CF488 (Green). The nuclear counterstain is Reddot (Red)
Western Blot Analysis of Colon tissue lysate using Calponin-1 Recombinant Rabbit Monoclonal Antibody (CNN1/1408R).
SDS-PAGE Analysis of Purified CNN1 Recombinant Rabbit Monoclonal Antibody (CNN1/1408R). Confirmation of Purity and Integrity of Antibody.
Western Blot analysis of Human Heart, Human Skeletal Muscle, and HEK293 lysates using Calponin-1 / CNN1 Recombinant Rabbit Monoclonal Antibody (CNN1/1408R).
Western Blot Analysis of Human Lung, Mouse Lung, Rat Lung, Hamster Lung, Guinea pig Lung, Rabbit Lung, Human Skeletal Muscle, Mouse Skeletal Muscle, Rat Skeletal Muscle, Hamster Skeletal Muscle, Guinea pig Skeletal Muscle, Rabbit Skeletal Muscle, Human Heart, Mouse Heart, Rat Heart, Hamster Heart, Guinea pig Heart and Rabbit Heart tissue lysates using Calponin-1 Recombinant Rabbit Monoclonal Antibody (CNN1/1408R).
Western Blot Analysis of Colon tissue lysate using Calponin-1 Recombinant Rabbit Monoclonal Antibody (CNN1/1408R).
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