Recombinant human FTH1 protein fragment (around aa 58-180) (exact sequence is proprietary)
Alternative Synonym:
Apoferritin, Cell proliferation-inducing gene 15 protein, Ferritin H subunit, Ferritin heavy chain, FHC, FRIH, FTH1, FTHL6, Iron overload autosomal dominant, PIG15, Placenta immunoregulatory factor, Proliferation inducing gene 15 protein (PLIF)
Mammalian ferritins consist of 24 subunits made up of 2 types of poly-peptide chains, ferritin heavy chain and ferritin light chain, which each have unique functions. Ferritin heavy chains catalyze the first step in iron storage, the oxidationof FeI(II), whereas ferritin light chains promote the nucleation of ferrihydrite, enabling storage of Fe(III). The most prominent role of mammalian ferritins is to provide iron-buffering capacity to cells. In addition to iron buffering, heavy chain ferritin is also involved in the regulation of thymidine biosynthesis via increased expression of cytoplasmic serine hydroxymethyltransferase, which is a limiting factor in thymidylate synthesis in MCF-7 cells. Light chain ferritin is involved in cataracts by at least two mechanisms: hereditary hyperferritinemia cataract syndrome, in which light chain ferritin is overexpressed, and oxidative stress, an important factor in the development of aging-related cataracts.
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Anwendungsbeschreibung:
Western Blot (1-2ug/ml), ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 2
Formal
Western Blot Analysis of HeLa cell lysate using Ferritin, Heavy Chain Mouse Monoclonal Antibody (FTH/2082).
SDS-PAGE Analysis of Purified Ferritin, Heavy Chain Mouse Monoclonal Antibody (FTH/2082). Confirmation of Purity and Integrity of Antibody.
Formalin-fixed, paraffin-embedded human pancreas stained with Ferritin, Heavy Chain Mouse Monoclonal Antibody (FTH/2082). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.
2495-MSM2-P1ABX
2495-MSM2-P1ABX
Western Blot Analysis of Human Brain, Mouse Brain, Rat Brain, Human Kidney, Mouse Kidney and Rat Kidney tissue lysates using Ferritin Heavy Chain Mouse Monoclonal Antibody (FTH/2082).
Western Blot Analysis of Human Brain, A-549 and A-431 lysate using Ferritin Heavy Chain Mouse Monoclonal Antibody (FTH/2082).
Western blot analysis of HeLa cell lysate using Ferritin Heavy Chain Rabbit Monoclonal Antibody (FTH/2082).
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
