Alkaline Phosphatase (Placental) / PLAP (Germ Cell Tumor Marker) Antibody, IgG1, Clone: [rALP/870], Mouse, Monoclonal

Produktübersicht

• Artikelname: Alkaline Phosphatase (Placental) / PLAP (Germ Cell Tumor Marker) Antibody, IgG1, Clone: [rALP/870], Mouse, Monoclonal
• Artikelnummer: NBT-250-MSM7-P1ABX
• Hersteller Artikelnummer: 250-MSM7-P1ABX
• Alternativnummer: NBT-250-MSM7-P1ABX-100
• Hersteller: NeoBiotechnologies
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: IHC, WB
• Spezies Reaktivität: Human
• Immunogen: Recombinant full-length human ALPP protein
• Alternative Synonym: Alkaline phosphatase placental type, Alkaline phosphatase Regan Isozyme, Alp1, ALPP, Germ-cell alkaline phosphatase, nagao Isozyme, PALP, Placental alkaline phosphatase 1, placental heat-stable alkaline phosphatase, PLAP1

Produktbeschreibung

Reacts with a 70kDa membrane-bound isozyme (Regan and Nagao type) of Placental Alkaline Phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is highly specific for PLAP and shows no cross-reaction with other isozymes of alkaline phosphatase. Anti-PLAP reacts with germ cell tumors and can discriminate between these and other neoplasms. Somatic neoplasms e.g. breast, gastrointestinal, prostatic, and urinary cancers may also immunoreact with antibodies to PLAP. Anti-PLAP positivity in conjunction with anti-keratin negativity favors seminoma over carcinoma. Germ cell tumors are usually anti-keratin positive, but they regularly fail to stain with anti-EMA, whereas most carcinomas stain with anti-EMA. Anti-PLAP has been useful in the diagnosis of gestational trophoblastic disease.

Produkteigenschaften

• Klonalität: Monoclonal
• Klon-Bezeichnung: [rALP/870]
• Molekulargewicht: 70kDa
• Isotyp: IgG1
• NCBI: 250
• UniProt: P05187
• Formulierung: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Antibody Type: Monoclonal Antibody

Anwendungsbeschreibung

• Anwendungsbeschreibung: Western Blot (1-2ug/ml),,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 &degC followed by cooling at RT for 2

Bilder

IHC analysis of formalin-fixed, paraffin-embedded human placenta stained using rALP/870 at 2ug/ml in PBS for 30min RT.

IHC analysis of formalin-fixed, paraffin-embedded human tonsil. Negative tissue control using rALP/870 at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.

IHC analysis of formalin-fixed, paraffin-embedded human testis. Negative tissue control using rALP/870 at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.

IHC analysis of formalin-fixed, paraffin-embedded human kidney. Negative tissue control using rALP/870 at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.

IHC analysis of formalin-fixed, paraffin-embedded human heart. Negative tissue control using rALP/870 at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.

IHC analysis of formalin-fixed, paraffin-embedded human colon. Negative tissue control using rALP/870 at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.

SDS-PAGE Analysis Purified Alkaline Phosphatase Recombinant Mouse Monoclonal (rALP/870). Confirmation of Purity and Integrity of Antibody.

Western Blot Analysis of human Plecenta tissue lysate using PLAP Recombinant Mouse Monoclonal Antibody (rALP/870).