Ku (p70/p80) (Nuclear Marker) Antibody, IgG1, Clone: [KU729], Mouse, Monoclonal

Produktübersicht

• Artikelname: Ku (p70/p80) (Nuclear Marker) Antibody, IgG1, Clone: [KU729], Mouse, Monoclonal
• Artikelnummer: NBT-2547-MSM1-P1ABX
• Hersteller Artikelnummer: 2547-MSM1-P1ABX
• Alternativnummer: NBT-2547-MSM1-P1ABX-100
• Hersteller: NeoBiotechnologies
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: FC, IF
• Spezies Reaktivität: Human
• Immunogen: Nuclear extract of human HL-60 cells
• Alternative Synonym: 70kDa / 80kDa subunit of Ku antigen, CTC box-binding factor 75kDa subunit, CTC75, CTCBF, G22P1, Lupus Ku autoantigen protein p70, ML8, Thyroid autoantigen 70kD (Ku antigen), Thyroid-lupus autoantigen (TLAA), X-ray repair cross-complementing protein 6 (XRCC6), ATP-dependent DNA helicase II 80kDa subunit, CTC box-binding factor 85kDa subunit, CTC85, CTCBF, KARP1, KUB2, Lupus Ku autoantigen protein p86, Nuclear factor IV (NFIV), X-ray repair cross-complementing protein 5 (XRCC5)

Produktbeschreibung

Recognizes a dimer of two proteins of 70kDa and ~80kDa, identified as two subunits of Ku. This MAb recognizes a conformational epitope of p70/p80 dimer, which is destroyed during Western blotting. The p70/p80 dimer is important for function of a 460kDa DNA-dependent protein kinase. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.

Produkteigenschaften

• Klonalität: Monoclonal
• Klon-Bezeichnung: [KU729]
• Molekulargewicht: 70kDa & 80kDa
• Isotyp: IgG1
• NCBI: 2547
• UniProt: P12956
• Formulierung: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Antibody Type: Monoclonal Antibody

Anwendungsbeschreibung

• Anwendungsbeschreibung: Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), ,Immunocytochemistry (Acetone-fixed cells) (0.5-1.0ug/ml for 30 minutes at RT),Optimal dilution for a specific application should be determined.

Bilder

Flow Cytometry of human Ku (p70/p80) on 293T cells. cells alone, Grey: isotype

Flow Cytometry of Human Ku (p70/p80) on 293T cells. Black: cells alone, Grey: Isotype Control, Green: CF488-labeled Ku Monoclonal Antibody (KU729).

Immunofluorescent analysis of PFA-fixed HeLa cells. Ku Mouse Monoclonal Antibody (KU729) followed by goat anti-mouse IgG-CF488 (green). Membrane stained with phalloidin (red).

Flow Cytometry of human Ku (p70/p80) on 293T cells. cells alone, Grey: isotype control, Green: AF488-labeled Ku Mouse Monoclonal Antibody (KU729).

Flow Cytometry of human Ku (p70/p80) on K562 cells. Black: cells alone, Green: isotype control, Red: PE-labeled Ku Mouse Monoclonal Antibody (KU729).

SDS-PAGE Analysis of Purified Ku Mouse Monoclonal Antibody (KU729). Confirmation of Purity and Integrity of Antibody.

Flow Cytometric analysis of human Ku (p70/p80) on K562 cells. Black: cells alone, Green: Isotype Control, Red: PE-labeled Ku Monoclonal Antibody (KU729).