PD-L1 / PDCD1LG1 / CD274 / B7-H1 (Cancer Immunotherapy Target) Antibody, IgG2b, Clone: [PDL1/2746], Mouse, Monoclonal

Produktübersicht

• Artikelname: PD-L1 / PDCD1LG1 / CD274 / B7-H1 (Cancer Immunotherapy Target) Antibody, IgG2b, Clone: [PDL1/2746], Mouse, Monoclonal
• Artikelnummer: NBT-29126-MSM6-P1
• Hersteller Artikelnummer: 29126-MSM6-P1
• Alternativnummer: NBT-29126-MSM6-P1-100
• Hersteller: NeoBiotechnologies
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: ELISA, FC, IF, IHC, WB
• Spezies Reaktivität: Human, Mouse
• Immunogen: Recombinant fragment (around aa 39-191) of human CD274 (PD-L1) protein (exact sequence is proprietary)
• Alternative Synonym: B7 homolog 1, B7-H1, CD274, PD-L1, PDCD1 ligand 1, PDCD1L1, PDCD1LG1, Programmed cell death 1 ligand 1

Produktbeschreibung

PD-L1 is a checkpoint regulator in immune cells, it is expressed on immune or non-hematopoietic cells. Expression of the protein is seen during pregnancy where it has a role in suppressing the immune system. PD-L1 induces an inhibitory signal in activated T-cells and promotes T-cell apoptosis. It is overexpressed in a number of different cancers where it is believed to play a significant role in the cancer s ability to evade the immune system.

Produkteigenschaften

• Klonalität: Monoclonal
• Klon-Bezeichnung: [PDL1/2746]
• Molekulargewicht: 37-50kDa
• Isotyp: IgG2b
• NCBI: 29126
• UniProt: Q9NZQ7
• Formulierung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Bilder

Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma stained with PD-L1-Monospecific Mouse Monoclonal Antibody (PDL1/2746). HIER: Tris/EDTA, pH9.0, 45min. 2°: HRP-polymer, 30min. DAB, 5min.

Analysis of Protein Array containing more than 19,000 full-length human proteins using PD-L1-Monospecific Mouse Monoclonal Antibody (PDL1/2746).Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD,Aos) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD,Aos) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Formalin-fixed, paraffin-embedded human cervical carcinoma stained with PD-L1-Monospecific Mouse Monoclonal Antibody (PDL1/2746). HIER: Tris/EDTA, pH9.0, 45min. 2°: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human breast carcinoma stained with PD-L1-Monospecific Mouse Monoclonal Antibody (PDL1/2746). HIER: Tris/EDTA, pH9.0, 45min. 2°: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human basal cell carcinoma stained with PD-L1-Monospecific Mouse Monoclonal Antibody (PDL1/2746). HIER: Tris/EDTA, pH9.0, 45min. 2°: HRP-polymer, 30min. DAB, 5min.

SDS-PAGE Analysis of Purified PD-L1 Mouse Monoclonal Antibody (PDL1/2746). Confirmation of Purity and Integrity of Antibody.

Flow cytometric analysis of Jurkat cells. PD-L1-Monospecific Mouse Monoclonal Antibody (PDL1/2746) followed by goat anti-Mouse IgG-CF488 (orange), cells alone (blue), isotype control (red).

Immunofluorescent staining of Jurkat cells. PD-L1-Monospecific Mouse Monoclonal Antibody (PDL1/2746) followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are stained with Reddot (Red)

Immunofluorescent staining of Jurkat cells. PD-L1-Monospecific Mouse Monoclonal Antibody (PDL1/2746) followed by goat anti-mouse IgG-CF488 (green). Nuclei are stained with RedDot (red).

Flow Cytometric Analysis of human Jurkat cells using PE-conjugated PD-L1 Mouse Monoclonal Antibody (PDL1/2746) (blue), isotype control (red).

SDS-PAGE Analysis of Purified PD-L1 Mouse Monoclonal Antibody (PDL1/2746) (unconjugated). Confirmation of Purity and Integrity of Antibody.