Recombinant fragment of human FOXA1 protein (aa372-472) (exact sequence is proprietary)
Alternative Synonym:
Forkhead box protein A1 (FOXA1), Hepatocyte nuclear factor 3-alpha (HNF-3-alpha or HNF-3A or HNF3A), Transcription factor 3A (TCF-3A or TCF3A)
The transcription factor Forkhead-box A1 (FOXA1), also known as hepatocyte nuclear factor 3-alpha, is a member of the FOX class of transcription factors.HNF-1 (), and HNF-6 compose, in part, a homeoprotein family designated the hepatocyte nuclear factor family. The various HNF-1 isoforms regulate transcription of genes in the liver as well as in other tissues such as kidney, small intestine and thymus. FOXA1 is expressed in normal breast ductal epithelium and other epithelium in different organs, such as lung, pancreas, bladder, prostate, and colon. Recently, FOXA1 has been shown to be a major determinant of estrogen-ER activity and endocrine response in breast cancer cells. FOXA1 expression correlates with estrogen receptor (ER)-positivity, especially in luminal subtype A breast cancers, which is associated with favorable prognosis. FOXA1 is useful in the sub-classification of breast carcinomas.
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Anwendungsbeschreibung:
Flow Cytometry (0.5-1ug/million cells), Immunofluorescence (1-2ug/ml), Western Blot (1-2ug/ml), Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1m
Formalin-fixed, paraffin-embedded Rat Colon stained with FOXA1 Monoclonal Antibody (FOXA1/1518).
Western Blot Analysis of human HeLa cell lysa
Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with FOXA1 Monoclonal Antibody (FOXA1/1518).
Formalin-fixed, paraffin-embedded Rat Colon stained with FOXA1 Monoclonal Antibody (FOXA1/1518).
Western Blot Analysis of human HeLa cell lysate using FOXA1 Monoclonal Antibody (FOXA1/1518).
SDS-PAGE Analysis Purified FOXA1 Monoclonal Antibody (FOXA1/1518). Confirmation of Integrity and Purity of Antibody.
Flow Cytometric Analysis of PFA fixed MCF-7 cells using FOXA1 Monoclonal Antibody (FOXA1/1518), followed by goat anti-mouse IgG-CF488 (Blue), Goat anti-mouse IgG-CF488 Is Control (Red).
Immunofluorescence Analysis of PFA-fixed MCF-7 cells labeled withFOXA1 Monoclonal Antibody (FOXA1/1518)followed by goat anti- mouse IgG-CF488. Counterstain is Phalloiden (Red).
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