60kDa heat shock protein mitochondrial, Chaperonin, 60-KD (CPN60), GROEL, HLD4, HSP65, HuCHA60, SPG13, Mitochondrial matrix protein P1, P60 lymphocyte protein, Short heat shock protein 60 Hsp60s1, Spastic paraplegia 13
Recognizes a 60kDa protein, identified as the heat shock protein 60 (hsp60). Its epitope is localized between aa 383-447 of human hsp60. A wide variety of environmental and pathophysiological stressful conditions trigger the synthesis of a family of proteins known as heat shock proteins (hsp s), more appropriately called as stress response proteins (srp s). hsp60 is a potential antigen in a number of autoimmune diseases. In human arthritis and in experimentally induced arthritis in animals, disease development coincides with the development of immune reactivity directed against not only bacterial hsp60, but also against its mammalian homolog. Clone rHSPD1/6497, unlike LK2, recognizes only the mammalian (not bacterial) hsp60 and is useful in distinguishing hsp60 from mammals and bacteria.
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.
Antibody Type:
Recombinant Monoclonal Antibody
Anwendungsbeschreibung:
Western Blot (1-2ug/ml), ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for
Western blot analysis of Hep-2 cell lysate using HSP60 Recombinant Mouse Monoclon
Formalin-fixed, paraffin-embedded human kidney stained with HSP60 Recombinant Mouse Monoclonal Antibody (rHSPD1/6497). Inset: 2°C Ab only control. Hematoxylin counterstain.
Western blot analysis of Hep-2 cell lysate using HSP60 Recombinant Mouse Monoclonal Antibody (rHSPD1/6497).
SDS-PAGE Analysis of Purified HSP60 Recombinant Mouse Monoclonal Antibody (rHSPD1/6497). Confirmation of Purity and Integrity of Antibody.
Western blot analysis of Hep-2 and HeLa cell lysate using HSP60 Recombinant Mouse Monoclonal Antibody (rHSPD1/6497).
Western Blot Analysis of HepG2 cell lysate using HSP60 Mouse Recombinant Monoclonal Antibody (rHSPD1/6497).
Western Blot Analysis of HepG2 and HeLa cell lysate using HSP60 Mouse Recombinant Monoclonal Antibody (rHSPD1/6497).
Western blot analysis of Human Brain, Human Kidney, Human Liver, Human Pancreas, A549, HeLa, Jurkat and K-562 lysates using HSP60 Recombinant Mouse Monoclonal Antibody (rHSPD1/6497).
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