CD11b is a cell adhesion molecule that acts as a receptor for cell surface ligands such as intracellular adhesion molecules (ICAMs) or soluble ligands. Integrins are heterodimeric proteins that contain an a chain and b chain. Integrin OO OOM combines with the Integrin 2 to form a leukocyte-specific integrin referred to as macrophage receptor 1 (Mac-1), or inactivated-C3b (iC3b) receptor 3 (CR3). Integrin OO OOM/ 2 is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. The protein CD11b has been implicated in the various adhesion-related interactions of cells such as monocytes, macrophages, natural killer (NK) cells, and granulocytes. It is part of a heterodimer that consists of CD11b andCD18. It also modulates the uptake of complement-coated particles within the cell. It is commonly used as a microglial marker in tissues derived from the nervous system.
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Anwendungsbeschreibung:
Western Blotting (1-2ug/ml),Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Formali
Western Blot Analysis of spleen tissue lysate using CD11b Monospecific Mouse Monoclonal Antibody (ITGAM/3338).
SDS-PAGE Analysis Purified CD11b Monospecific Mouse Monoclonal Antibody (ITGAM/3338). Confirmation of Purity and Integrity of Antibody.
Formalin-fixed, paraffin-embedded human tonsil stained with CD11b Monospecific Mouse Monoclonal Antibody (ITGAM/3338).
Formalin-fixed, paraffin-embedded human tonsil stained with CD11b Monospecific Mouse Monoclonal Antibody (ITGAM/3338).
Analysis of Protein Array containing more than 19,000 full-length human proteins using CD11b Monospecific Mouse Monoclonal Antibody (ITGAM/3338). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD&apos,s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD&apos,s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Western Blot analysis of Human Lung, Mouse Lung, Rat Lung, Hamster Lung, Guinea pig Lung, and Rabbit Lung tissue lysates using CD11b Mouse Monoclonal Antibody (ITGAM/3338).
Western Blot Analysis of Human Lung tissue lysate using CD11b Mouse Monoclonal Antibody (ITGAM/3338).
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