Recombinant EpCAM / CD326 (Epithelial Marker) Antibody, Clone: [EGP40/2041R], Rabbit, Monoclonal

Produktübersicht

• Artikelname: Recombinant EpCAM / CD326 (Epithelial Marker) Antibody, Clone: [EGP40/2041R], Rabbit, Monoclonal
• Artikelnummer: NBT-4072-RBM21-P0
• Hersteller Artikelnummer: 4072-RBM21-P0
• Alternativnummer: NBT-4072-RBM21-P0-20,NBT-4072-RBM21-P0-100
• Hersteller: NeoBiotechnologies
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: IHC, WB
• Spezies Reaktivität: Canine, Feline, Guinea pig, Human
• Immunogen: Recombinant fragment from the extracellular domain of human EpCAM protein (around aa100-224) (exact sequence is proprietary)
• Alternative Synonym: Adenocarcinoma-associated Antigen, Cell Surface Glycoprotein Trop-1, EGP2, EGP314, EGP40, Epithelial Cell Adhesion Molecule, Epithelial Glycoprotein 314, ESA, KSA, TACD1, TROP1, Tumor-associated Calcium Signal Transducer 1 (TACSTD1)

Produktbeschreibung

Epitope of this MAb is mapped between aa 202-212 of EGP40, which is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. Antibody to Ep-CAM has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

Produkteigenschaften

• Klonalität: Monoclonal
• Klon-Bezeichnung: [EGP40/2041R]
• Molekulargewicht: 40-43kDa
• NCBI: 4072
• UniProt: P16422
• Formulierung: 200ug/ml of Ab purified by Protein A. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also availableWITHOUT BSA & azide at 1.0mg/ml.
• Antibody Type: Recombinant Monoclonal Antibody

Anwendungsbeschreibung

• Anwendungsbeschreibung: Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 &degC followed by cooling at RT for 20 minutes),Optimal diluti

Bilder

Formalin-fixed, paraffin-embedded human basal cell carcinoma stained with EpCAM Recombinant Rabbit Monoclonal Antibody (EGP40/2041R).

Formalin-fixed, paraffin-embedded canine (dog) bladder stained with EpCAM Recombinant Rabbit Monoclonal Antibody (EGP40/2041R).

Formalin-fixed, paraffin-embedded feline (cat) small intestine stained with EpCAM Recombinant Rabbit Monoclonal Antibody (EGP40/2041R).

Formalin-fixed, paraffin-embedded human thyroid carcinoma stained with EpCAM Recombinant Rabbit Monoclonal Antibody (EGP40/2041R).

SDS-PAGE Analysis Purified EpCAM Recombinant Rabbit Monoclonal Antibody (EGP40/2041R). Confirmation of Purity and Integrity of Antibody.

Western blot analysis of MCF-7 cell lysate using EpCAM Recombinant Rabbit Monoclonal Antibody (EGP40/2041R).

Western blot analysis of human lung tissue lysate using EpCAM Recombinant Rabbit Monoclonal Antibody (EGP40/2041R).

Western blot analysis of Human Lung, Mouse Lung, Rat Lung, Hamster Lung, Guinea pig Lung, Human Intestine, Mouse Intestine, Rat Intestine, Hamster Intestine and Guinea pig Intestine tissue lysates using EpCAM Recombinant Rabbit Monoclonal Antibody (EGP40/2041R).

Western blot analysis of HEK293, HCT116, and MCF7 cell lysates using EpCAM Recombinant Rabbit Monoclonal Antibody (EGP40/2041R).