Recombinant human Ki67 protein fragment (around aa 2293-2478) (exact sequence is proprietary)
Alternative Synonym:
KI-67, Ki67, KI-67 Antigen (KIA), MKI67, Proliferation related Ki-67 antigen
Ki-67 antigen is a nuclear, non-histone protein that is present in all stages of the cell cycle except G0. This characteristic makes Ki-67 an excellent marker for proliferating cells and is commonly used as one of the prognostic factors in cancer studies. A correlation has been demonstrated between Ki-67 index and the histo-pathological grade of neoplasms. Assessment of Ki-67 expression in renal and ureter tumors shows a correlation between tumor proliferation and disease progression, thus making it possible to differentiate high-risk patients. Ki-67 expression may also prove to be important for distinguishing between malignant and benign peripheral nerve sheath tumors. Ki-67 labeling index has been shown to be a prognostic marker in a number of neoplasms including grade II astrocytoma, oligodendroglioma, colon carcinoma, and breast carcinoma. In general, Ki-67 is a good marker of proliferating cell populations.
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Anwendungsbeschreibung:
Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Western Blot (1-2ug/ml), ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1m
Formalin-fixed, pa
Formalin-fixed, paraffin-embedded human tonsil stained with Ki67-Monospecific Mouse Monoclonal Antibody (MKI67/2462).
Formalin-fixed, paraffin-embedded human tonsil stained with Ki67-Monospecific Mouse Monoclonal Antibody (MKI67/2462).
Formalin-fixed, paraffin-embedded human tonsil-skin stained with Ki67-Monospecific Mouse Monoclonal Antibody (MKI67/2462).
SDS-PAGE Analysis Purified Ki67-Monospecific Mouse Monoclonal Antibody (MKI67/2462).Confirmation of Purity and Integrity of Antibody.
Immunofluorescent staining of HeLa cells. Ki67-Monospecific Mouse Monoclonal Antibody (MKI67/2462) followed by goat anti-mouse IgG-CF488 (green). Membrane stained red (phalloidin).
Flow Cytometric Analysis of HeLa cells. Ki67-Monospecific Mouse Monoclonal Antibody (MKI67/2462) followed by goat anti-mouse IgG-CF488 (blue), isotype control (red).
Western blot analysis of Raji and Ramos cell lysates using Ki67-Monospecific Mouse Monoclonal Antibody (MKI67/2462).
Analysis of Protein Array containing more than 19,000 full-length human proteins using Ki67-Monospecific Mouse Monoclonal Antibody (MKI67/2462). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD&apos,s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD&apos,s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
