Nucleolin (Marker of Human Cells) Antibody, IgG1, Clone: [364-5 + NCL/902], Mouse, Monoclonal

Produktübersicht

• Artikelname: Nucleolin (Marker of Human Cells) Antibody, IgG1, Clone: [364-5 + NCL/902], Mouse, Monoclonal
• Artikelnummer: NBT-4691-MSM3-P1
• Hersteller Artikelnummer: 4691-MSM3-P1
• Alternativnummer: NBT-4691-MSM3-P1-100
• Hersteller: NeoBiotechnologies
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: FC, IF, IHC, WB
• Spezies Reaktivität: Human
• Immunogen: Lysate of SU-DHL-1 Nuclei (364-5), Recombinant human NCL protein (NCL/902)
• Alternative Synonym: NCL, Nucl, Nucleolin, Protein C23

Produktbeschreibung

Recognizes a protein of ~76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.This MAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.

Produkteigenschaften

• Klonalität: Monoclonal
• Klon-Bezeichnung: [364-5 + NCL/902]
• Molekulargewicht: 76kDa
• Isotyp: IgG1
• NCBI: 4691
• UniProt: P19338
• Formulierung: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Bilder

Immunofluorescence Analysis of PFA-fixed MCF-

Immunofluorescence Analysis of PFA-fixed K562 cells stained with Nucleolin Mouse Monoclonal Antibody (364-5 + NCL/902) followed by goat anti-mouse IgG-CF488 (green). Membrane is stained with Phalloiden-CF640.

Immunofluorescence Analysis of PFA-fixed MCF-7 cells stained with Nucleolin Mouse Monoclonal Antibody (364-5 + NCL/902) followed by goat anti-mouse IgG-CF488 (green). Membrane is stained with Phalloiden-CF640.

Formalin-fixed, paraffin-embedded human colon stained with AF488 labeled Nucleolin Mouse Monoclonal Antibody (364-5 + NCL/902).

Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with Nucleolin Mouse Monoclonal Antibody (364-5 + NCL/902).

Flow Cytometric Analysis of PFA-fixed K562 cells. Nucleolin Mouse Monoclonal Antibody (364-5 + NCL/902) followed by goat anti-mouse IgG-CF488 (blue), isotype control (red).

Formalin-fixed, paraffin-embedded human tonsil stained with Nucleolin Mouse Monoclonal Antibody (364-5 + NCL/902).

Formalin-fixed, paraffin-embedded human testicular carcinoma stained with Nucleolin Mouse Monoclonal Antibody (364-5 + NCL/902).

Formalin-fixed, paraffin-embedded human uterus stained with Nucleolin Mouse Monoclonal Antibody (364-5 + NCL/902).