CD73 (Immuno-Oncology Target) Antibody, IgG1, Clone: [NT5E/2503], Mouse, Monoclonal

Produktübersicht

• Artikelname: CD73 (Immuno-Oncology Target) Antibody, IgG1, Clone: [NT5E/2503], Mouse, Monoclonal
• Artikelnummer: NBT-4907-MSM1-P1
• Hersteller Artikelnummer: 4907-MSM1-P1
• Alternativnummer: NBT-4907-MSM1-P1-100
• Hersteller: NeoBiotechnologies
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: FC, IF, IHC, WB
• Spezies Reaktivität: Human
• Immunogen: Recombinant full length human NT5E protein
• Alternative Synonym: 5 NT, 5 nucleotidase (CD73), 5 nucleotidase precursor, 5 nucleotidase, ecto, 5 nucleotidase, ecto (CD73), 5-NT, 5-nucleotidase, 5NTD_HUMAN, CD73, CD73 antigen, E5NT, Ecto 5 nucleotidase, Ecto-5-nucleotidase, eN, eNT, NT, NT5, NT5E, NTE, Purine 5 Prime Nucleotidase

Produktbeschreibung

CD73 (also designated ecto-5-nucleotidase, E5NT, NT, NT5, NTE, eN and eNT) is a glycosyl-phosphatidylinositol (GPI)-anchored adhesion protein that catalyzes the dephosphorylation of extracellular purine and pyrimidine nucleotides to their corresponding bioactive nucleosides. CD73 is a dimer of two identical subunits that depends on GPI to link with the external face of the plasma membrane. Similar to other GPI-anchored proteins, CD73 mediates co-stimulatory signals in T cell activation. CD73 has few structural variants, yet elicits diverse biological function through differential regulation in endothelial cells (EC), subpopulations of B and T cells, germinal center follicular dendritic cells and on thymic medullary reticular fibroblasts. For example, IgG mediated neutralization of CD73 interferes with lymphocyte adhesion to EC, and blocks aggregation of germinal center B cells and follicular dendritic cells. Furthermore, IgG-mediated targeting of lymphocyte CD73, but not of endothelial cell CD73, causes shedding of CD73 and tyrosine phosphorylation of proteins.

Produkteigenschaften

• Klonalität: Monoclonal
• Klon-Bezeichnung: [NT5E/2503]
• Molekulargewicht: 71kDa
• Isotyp: IgG1
• NCBI: 4907
• UniProt: P21589
• Formulierung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Bilder

Formalin-fixed, paraffin-embedded huma

Formalin-fixed, paraffin-embedded human Tonsil stained with CD73 Mouse Monoclonal Antibody (NT5E/2503).

Formalin-fixed, paraffin-embedded human Lung Carcinoma stained with CD73 Mouse Monoclonal Antibody (NT5E/2503).

Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with CD73 Mouse Monoclonal Antibody (NT5E/2503).

Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with CD73 Mouse Monoclonal Antibody (NT5E/2503).

Immunofluorescence staining of U87MG cells using CD73 Mouse Monoclonal Antibody (NT5E/2503) followed by goat anti-Mouse IgG conjugated to CF488 (green). Membrane stained with Phalloidin (Red).

Flow Cytometric Analysis of U87MG cells using CD73 Mouse Monoclonal Antibody (NT5E/2503) followed by goat anti-Mouse IgG-CF488 (Blue), Isotype Control (Red).

Western Blot Analysis of A431 and PC-3 cell lysate using CD73 Mouse Monoclonal Antibody (NT5E/2503).

SDS-PAGE Analysis of Purified CD74 Mouse Monoclonal Antibody (NT5E/2503). Confirmation of Purity and Integrity of Antibody.

Western Blot Analysis of Human Liver, Human Skin, and Human Spleen tissue lysates using CD73 Mouse Monoclonal Antibody (NT5E/2503).

Western blot analysis of A-431 and PC3 cell lysate using CD73 Mouse Monoclonal Antibody (NT5E/2503).