Recombinant fragment (around aa 148-255) of human BOB1 (POU2AF1) protein (exact sequence is proprietary)
Alternative Synonym:
B cell Oct binding protein 1, B-cell-specific coactivator OBF-1, BOB-1, OBF1, OCA-B, OCAB, OCT-binding factor 1, POU domain class 2-associating factor 1, POU2AF1
BOB.1 expression in a variety of established B-cell lines, representing different stages of B-cell development, has suggested a constitutive, B-cell-specific expression pattern. LP cells in nodular lymphocyte predominant Hodgkin lymphoma, because they are germinal center-derived, are consistently immuno-positive for BOB.1. Conversely, only some cases of classical Hodgkin lymphoma show BOB.1 immuno-reactivity within the Hodgkin and Reed-Sternberg cells.vC OOExpression of BOB.1 has been reported in follicular center cell lymphoma, diffuse large B-cell lymphoma and some cases of acute myeloid leukemia.vC OOB-CLL, marginal zone lymphoma, and mantle cell lymphoma may show weak to moderate immunoreactivity.
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Anwendungsbeschreibung:
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific
Formalin-fixed, paraffin-embedded human tonsil stained with BOB1 Mouse Monoclonal Antibody (BOB1/7469). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.
Formalin-fixed, paraffin-embedded human tonsil stained with BOB1 Mouse Monoclonal Antibody (BOB1/7469). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.
IHC analysis of formalin-fixed, paraffin-embedded human skin in tonsil. Negative tissue control using BOB1/7469 at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.
SDS-PAGE Analysis of Purified BOB1 Mouse Monoclonal Antibody (BOB1/7469). Confirmation of Purity and Integrity of Antibody.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
