B2M, Beta 2 microglobin, Beta 2 microglobulin, Beta chain of MHC class I molecules, Beta-2-microglobulin form pI 5.3, Hdcma22p
Recognizes a protein of 12kDa, identified as beta-2 microglobulin. Major histocompatibility complex (MHC) class 1 molecules bind to antigens for presentation on the surface of cells. The proteasome is responsible for producing these antigens from the components of foreign pathogens. MHC class 1 molecules consist of an alpha heavy chain that contains three subdomains (alpha1, alpha2, alpha3) and a non-covalent associating light chain, known as beta-2-Microglobulin. Beta-2-Microglobulin associates with the alpha3 subdomain of the alpha heavy chain and forms an immunoglobulin domain-like structure that mediates proper folding and expression of MHC class 1 molecules. The alpha1 and alpha2 domains of the alpha heavy chain form the peptide antigen-binding cleft. Mutations in the beta-2-Microglobulin gene can enhance the progression of malignant melanoma phenotypes.
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Recombinant Monoclonal Antibody
Anwendungsbeschreibung:
Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Western Blot (1-2ug/ml), Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM
Formalin-fixed, paraffin-embedded human Lung Carcinoma stained with Beta-2-Microglobulin Mouse Recombinant Monoclonal Antibody (rB2M/961).
Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Beta-2-Microglobulin Mouse Recombinant Monoclonal Antibody (rB/961).
Formalin-fixed, paraffin-embedded human Cervical Carcinoma stained with Beta-2-Microglobulin Mouse Recombinant Monoclonal Antibody (rB2M/961).
Western Blot Analysis of human kidney tissue lysates using Beta-2-Microglobulin mouse monoclonal antibody (B2M/1118).
SDS-PAGE Analysis Beta-2-Microglobulin Mouse Recombinant Monoclonal Antibody (rB2M/961). Confirmation of Purity and Integrity of Antibody.
Flow Cytometric Analysis of PFA-fixed HeLa cells using Beta-2-Microglobulin Mouse Recombinant MAb (rB2M/961) followed by Goat anti-Mouse IgG-CF488 (Blue), Isotype Control (Red)
Analysis of Protein Array containing more than 19,000 full-length human proteins using Beta-2 Microglobulin Mouse Recombinant Monoclonal Antibody (rB2M/961). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD&apos,s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD&apos,s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to be specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Western Blot Analysis of Human Spleen, Mouse Spleen, Rat Spleen, Human Lungs, Mouse Lungs and Rat Lungs tissue lysates using Beta-2-Microglobulin Mouse Recombinant Monoclonal Antibody (rB2M/961).
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