CD45 / LCA (B-Cell Marker) Antibody, IgG2a, Clone: [PTPRC/1461], Mouse, Monoclonal

Produktübersicht

• Artikelname: CD45 / LCA (B-Cell Marker) Antibody, IgG2a, Clone: [PTPRC/1461], Mouse, Monoclonal
• Artikelnummer: NBT-5788-MSM21-P1ABX
• Hersteller Artikelnummer: 5788-MSM21-P1ABX
• Alternativnummer: NBT-5788-MSM21-P1ABX-100
• Hersteller: NeoBiotechnologies
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: FC, IF, IHC
• Spezies Reaktivität: Human
• Immunogen: Human B-lymphocytes
• Alternative Synonym: B220, CD45R, GP180, Leukocyte common antigen (LCA), Loc, Ly-5, Lyt-4, Protein tyrosine phosphatase receptor type C (PTPRC), Receptor-type tyrosine-protein phosphatase C, T200 glycoprotein

Produktbeschreibung

CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45R functions as a phosphor-tyrosine phosphatase. Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms.

Produkteigenschaften

• Klonalität: Monoclonal
• Klon-Bezeichnung: [PTPRC/1461]
• Molekulargewicht: 180-220kDa
• Isotyp: IgG2a
• NCBI: 5788
• UniProt: P08575
• Formulierung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Antibody Type: Monoclonal Antibody

Anwendungsbeschreibung

• Anwendungsbeschreibung: Flow Cytometry (0.5-1ug/million cells), Immunofluorescence (0.5-1ug/ml), ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 4

Bilder

Formalin-fixed, paraffin-embedded human tonsil stained with CD45 Mouse Monoclonal Antibody (PTPRC/1461).

Immunofluorescence Analysis of PFA-fixed Jurkat cells labeling CD45RA. CD45 Mouse Monoclonal Antibody (PTPRC/1461) followed by goat anti-mouse IgG-CF488 (green). Nuclear stain is RedDot.

Flow Cytometric Analysis of PFA-fixed Jurkat cells. CD45 Mouse Monoclonal Antibody (PTPRC/1461) followed by goat anti-mouse IgG-CF488 (blue), isotype control (red).

SDS-PAGE Analysis of Purified CD45 Mouse Monoclonal Antibody (PTPRC/1461) Confirmation of Integrity and Purity of Antibody.