CD45RO (T-Cell Marker) Antibody, IgG2a, Clone: [T200/797], Mouse, Monoclonal

Produktübersicht

• Artikelname: CD45RO (T-Cell Marker) Antibody, IgG2a, Clone: [T200/797], Mouse, Monoclonal
• Artikelnummer: NBT-5788-MSM7-P0
• Hersteller Artikelnummer: 5788-MSM7-P0
• Alternativnummer: NBT-5788-MSM7-P0-20,NBT-5788-MSM7-P0-100
• Hersteller: NeoBiotechnologies
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: FC, IF, IHC
• Spezies Reaktivität: Human
• Immunogen: Human T-lymphocytes
• Alternative Synonym: T200, GP180, LCA, Leukocyte Common Antigen, LY5, Receptor-type tyrosine-protein phosphatase C, T200 glycoprotein

Produktbeschreibung

Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO). This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.

Produkteigenschaften

• Klonalität: Monoclonal
• Klon-Bezeichnung: [T200/797]
• Molekulargewicht: 180-185kDa
• Isotyp: IgG2a
• NCBI: 5788
• UniProt: P08575
• Formulierung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Antibody Type: Monoclonal Antibody

Anwendungsbeschreibung

• Anwendungsbeschreibung: Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45

Bilder

Flow Cytometric Analysis of PFA-fixe

Formalin-fixed, paraffin-embedded human Tonsil stained with CD45RO Mouse Monoclonal Antibody (T200/797).

SDS-PAGE Analysis CD45RO Mouse Monoclonal Antibody (T200/797). Confirmation of Purity and Integrity of Antibody.

Flow Cytometric Analysis of PFA-fixed Jurkat cells using CD45RO Mouse Monoclonal Antibody (T200/797) followed by Goat anti- Mouse- IgG-CF488 (Blue), Isotype Control (Red).