CD45RA (Leucocyte Marker) Antibody, IgG1, Clone: [SPM504], Mouse, Monoclonal

Produktübersicht

• Artikelname: CD45RA (Leucocyte Marker) Antibody, IgG1, Clone: [SPM504], Mouse, Monoclonal
• Artikelnummer: NBT-5788-MSM8X-P1ABX
• Hersteller Artikelnummer: 5788-MSM8X-P1ABX
• Alternativnummer: NBT-5788-MSM8X-P1ABX-100
• Hersteller: NeoBiotechnologies
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: FC, IF, IHC, WB
• Spezies Reaktivität: Human
• Immunogen: Stimulated human leukocytes
• Alternative Synonym: B220, CD45R, GP180, Leukocyte common antigen (LCA), Loc, Ly-5, Lyt-4, Protein tyrosine phosphatase receptor type C (PTPRC), Receptor-type tyrosine-protein phosphatase C, T200 glycoprotein

Produktbeschreibung

Recognizes a protein of 205kDa-220kDa, identified as CD45RA (Workshop III). CD45RA is isoforms of the human leukocyte common antigen (CD45). Human CD45 contains three exons which encode peptide segments designated A, B and C, respectively. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B and O. This antibody reacts with ABC and BC isoforms. CD45RA is expressed on 40-50% of peripheral CD4+ T-cells, 50% of peripheral CD8+ T-cells, B-cells, and leukemic B-cell lines. T-cells expressing CD45RA are naive or virgin T-cells. T-cells expressing CD45RO are memory T-cells. CD45RA and CD45RO define complementary, predominantly non-overlapping populations of resting peripheral T-cells. This MAb is useful in study on the subpopulation of CD4+ or CD8+ T-cells. It can especially be used to differentiate T-cell lymphomas (CD45RO +ve) from B cell lymphomas (CD45RA +ve).

Produkteigenschaften

• Klonalität: Monoclonal
• Klon-Bezeichnung: [SPM504]
• Molekulargewicht: 205-220kDa
• Isotyp: IgG1
• NCBI: 5788
• UniProt: P08575
• Formulierung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Antibody Type: Monoclonal Antibody

Anwendungsbeschreibung

• Anwendungsbeschreibung: Flow Cytometry (1-2ug/million cells), Western Blot (1-2ug/ml), ,Immunofluorescence (1-2ug/ml), ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1

Bilder

Formalin-fixed, paraffin-embedded human Tonsil stained with CD45RA Monoclonal Antibody (SPM504).

Western Blot Analysis of human spleen tissue lysates using CD45RA Monoclonal Antibody (SPM568).

Formalin-fixed, paraffin-embedded human Tonsil stained with CD45RA Monoclonal Antibody (SPM504).

Western Blot Analysis of human spleen tissue lysates using CD45RA Monoclonal Antibody (SPM568).