Recombinant CD45RA / LCA (B-Cell Marker) Antibody, Clone: [PTPRC/7019R], Rabbit, Monoclonal

Produktübersicht

• Artikelname: Recombinant CD45RA / LCA (B-Cell Marker) Antibody, Clone: [PTPRC/7019R], Rabbit, Monoclonal
• Artikelnummer: NBT-5788-RBM42-P0
• Hersteller Artikelnummer: 5788-RBM42-P0
• Alternativnummer: NBT-5788-RBM42-P0-20,NBT-5788-RBM42-P0-100
• Hersteller: NeoBiotechnologies
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: IHC, WB
• Spezies Reaktivität: Human
• Immunogen: Recombinant full-length human PTPRC protein
• Alternative Synonym: B220, CD45R, GP180, Leukocyte common antigen (LCA), Loc, Ly-5, Lyt-4, Protein tyrosine phosphatase receptor type C (PTPRC), Receptor-type tyrosine-protein phosphatase C, T200 glycoprotein

Produktbeschreibung

CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45R functions as a phosphor-tyrosine phosphatase. Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms.

Produkteigenschaften

• Klonalität: Monoclonal
• Klon-Bezeichnung: [PTPRC/7019R]
• Molekulargewicht: 180-220kDa
• NCBI: 5788
• UniProt: P08575
• Formulierung: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Antibody Type: Recombinant Monoclonal Antibody

Anwendungsbeschreibung

• Anwendungsbeschreibung: Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 &degC followed by cooling at RT for 20 minutes),Optimal diluti

Bilder

SDS-PAGE Analysis of Purified CD45RA Recombinant Rabbit Mono

Formalin-fixed, paraffin-embedded human tonsil stained with CD45RA Recombinant Rabbit Monoclonal Antibody (PTPRC/7019R). Inset: PBS instead of primary antibody, secondary only negative control.

SDS-PAGE Analysis of Purified CD45RA Recombinant Rabbit Monoclonal Antibody (PTPRC/7019R). Confirmation of Purity and Integrity of Antibody.

Western blot analysis of Raji and Ramos cell lysates using CD45RA / LCA Rabbit Recombinant Monoclonal Antibody (PTPRC/7019R).