vü-Actin (ACTB) Antibody, Clone: [ACTB/2370], Mouse, Monoclonal

Produktübersicht

• Artikelname: vü-Actin (ACTB) Antibody, Clone: [ACTB/2370], Mouse, Monoclonal
• Artikelnummer: NBT-60-MSM4-P0
• Hersteller Artikelnummer: 60-MSM4-P0
• Alternativnummer: NBT-60-MSM4-P0-20,NBT-60-MSM4-P0-100
• Hersteller: NeoBiotechnologies
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: IHC
• Spezies Reaktivität: Human
• Immunogen: Recombinant fragment (around aa150-350) of human ACTB protein (exact sequence is proprietary)
• Alternative Synonym: A26C1A, A26C1B, ACTB, Actin beta, Actin, cytoplasmic 1, N-terminally processed, Actx, b actin, Beta cytoskeletal actin, Beta-actin, BRWS1, E430023M04Rik, Melanoma X actin, MGC128179, PS1TP5 binding protein 1, PS1TP5BP1

Produktbeschreibung

All eukaryotic cells express Actin, which often constitutes as much as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one Actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of Actin are present in mammalian tissues and fall into three classes. a-Actin expression is limited to various types of muscle, whereas ve OO-Actin and g-Actin are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the Actin cytoskeleton. Rho controls the assembly of Actin stress fibers and focal adhesion. Rac regulates Actin filament accumulation at the plasma membrane. Cdc42 stimulates formation of filopodia.

Produkteigenschaften

• Klonalität: Monoclonal
• Klon-Bezeichnung: [ACTB/2370]
• Molekulargewicht: 43kDa
• NCBI: 60
• UniProt: P60709
• Formulierung: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Antibody Type: Monoclonal Antibody

Anwendungsbeschreibung

• Anwendungsbeschreibung: Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes),Optimal dilutio

Bilder

SDS-PAGE Analysis of Purified Beta Actin Mouse Monoclonal (ACTB/2370). Confirmation of Purity and Integrity of Antibody.

Formalin-fixed, paraffin-embedded human spleen stained with Beta Actin Mouse Monoclonal (ACTB/2370). Inset: PBS instead of primary antibody, secondary only negative control.