Recombinant S100A4 / Metastasin / Calvasculin (Marker of Tumor Metastasis) Antibody, IgG1, Clone: [rS100A4/1481], Mouse, Monoclonal

Produktübersicht

• Artikelname: Recombinant S100A4 / Metastasin / Calvasculin (Marker of Tumor Metastasis) Antibody, IgG1, Clone: [rS100A4/1481], Mouse, Monoclonal
• Artikelnummer: NBT-6275-MSM5-P0
• Hersteller Artikelnummer: 6275-MSM5-P0
• Alternativnummer: NBT-6275-MSM5-P0-20,NBT-6275-MSM5-P0-100
• Hersteller: NeoBiotechnologies
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: FC, IF, IHC, WB
• Spezies Reaktivität: Human, Mouse
• Immunogen: Recombinant fragment (around aa 1-200) of human S100A4 protein (exact sequence is proprietary)
• Alternative Synonym: S100 calcium-binding protein A4, CAPL, Fibroblast specific protein 1 (FSP1), Leukemia multidrug resistance associated protein, Malignant transformation suppression 1 (MTS1), Metastasin, Placental calcium-binding protein

Produktbeschreibung

S100A4 belongs to the S100 super-family of proteins containing 2 EF-hand calcium-binding domains. S100 genes include at least 25 members, including S100A1-S100A18, trichohyalin, filaggrin, repetin, S100P, and S100Z. S100A4 exerts its function via direct interaction with a number of proteins including P53, P63, non-muscle myosin IIA, 64 integrin, and liprin b1. S100A4 is overexpressed in highly metastatic cancers, which makes it useful as a marker of tumor progression.

Produkteigenschaften

• Klonalität: Monoclonal
• Klon-Bezeichnung: [rS100A4/1481]
• Molekulargewicht: 10-12kDa
• Isotyp: IgG1
• NCBI: 6275
• UniProt: P26447
• Formulierung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Antibody Type: Recombinant Monoclonal Antibody

Anwendungsbeschreibung

• Anwendungsbeschreibung: Flow Cytometry (1-2ug/million cells), Western Blot (1-2ug/ml), Immunofluorescence (1-2ug/ml),,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM

Bilder

Flow Cyt

Formalin-fixed, paraffin-embedded human Placenta stained with S100A4 Recombinant Mouse Monoclonal Antibody (rS100A4/1481).

Formalin-fixed, paraffin-embedded human Placenta stained with S100A4 Recombinant Mouse Monoclonal Antibody (rS100A4/1481).

Flow Cytometric Analysis of A549 cells using S100A4 Recombinant Mouse Monoclonal Antibody (rS100A4/1481) followed by goat anti-Mouse IgG-CF488 (Blue), Isotype Control (Red).

Immunofluorescence staining of A549 cells using S100A4 Recombinant Mouse Monoclonal Antibody (rS100A4/1481) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot.

SDS-PAGE Analysis Purified S100A4 Recombinant Mouse Monoclonal Antibody (rS100A4/1481). Confirmation of Purity and Integrity of Antibody.

Western Blot Analysis of Panc-1 cell lysate using S100A4 Recombinant Mouse Monoclonal Antibody (rS100A4/1481).

Western Blot Analysis of Human Heart, Human Lung, Human Spleen, A549, and HeLa lysates using S100A4 Recombinant Mouse Monoclonal Antibody (rS100A4/1481).

Western Blot Analysis of HeLa and A549 lysates using S100A4 Recombinant Mouse Monoclonal Antibody (rS100A4/1481).