Recombinant Spectrin beta III (SPTBN2) Antibody, Clone: [SPTBN2/2887R], Rabbit, Monoclonal

Produktübersicht

• Artikelname: Recombinant Spectrin beta III (SPTBN2) Antibody, Clone: [SPTBN2/2887R], Rabbit, Monoclonal
• Artikelnummer: NBT-6712-RBM6-P1ABX
• Hersteller Artikelnummer: 6712-RBM6-P1ABX
• Alternativnummer: NBT-6712-RBM6-P1ABX-100
• Hersteller: NeoBiotechnologies
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: FC, IF, IHC, WB
• Spezies Reaktivität: Human
• Immunogen: Recombinant human SPTBN2 fragment (aa356-475) (exact sequence is proprietary)
• Alternative Synonym: Beta III spectrin, SCA5, Spectrin beta chain brain 2, Spectrin beta non-erythrocytic 2, Spectrin non-erythroid beta chain 2, Spinocerebellar ataxia 5, SPTBN2

Produktbeschreibung

Spectrin is an actin binding protein that is a major component of the plasma membrane skeleton. Spectrins function as membrane organizers and stabilizers by forming dimers, tetramers and higher polymers. Vertebrate spectrins have two alpha-subunits (alpha-I/alpha-II) four beta-subunits (beta-I-beta-IV) and a beta-H subunit creating diversity and specialization of function. Spectrin III is highly expressed in brain, kidney, pancreas and liver, and at lower levels in lung and placenta. Spectrin beta 3 is primarily expressed in nervous tissues with highest expression levels in the cerebellum, where it is found in Purkinje cell soma and dendrites.

Produkteigenschaften

• Klonalität: Monoclonal
• Klon-Bezeichnung: [SPTBN2/2887R]
• Molekulargewicht: 246kDa
• NCBI: 6712
• UniProt: O15020
• Formulierung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Antibody Type: Recombinant Monoclonal Antibody

Anwendungsbeschreibung

• Anwendungsbeschreibung: Flow Cytometry (1-2ug/million cells), (Immunofluorescence (1-2ug/ml), Western Blot (1-2ug/ml), Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1m

Bilder

Western Blot Analysis of PANC-1 cell lysate using Spectrin beta III Rabbit Recombinant Monoclonal (SPTBN2/2887R).

SDS-PAGE Analysis of Purified Spectrin beta III Rabbit Recombinant Monoclonal (SPTBN2/2887R). Confirmation of Purity and Integrity of Antibody.

Immunofluorescence Analysis of Human HeLa cells labeling SPTBN2 with Spectrin beta III Rabbit Recombinant Monoclonal (SPTBN2/2887R) followed by Goat anti-mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).

Flow Cytometric Analysis of HeLa cells using Spectrin beta III Rabbit Recombinant Monoclonal Antibody (SPTBN2/2887R) followed by Goat anti-rabbit IgG-CF488 (Blue), Isotype Control (Red).

Western Blot Analysis of Human Kidney, Human Testis and A549 lysates using Spectrin Beta 3 Recombinant Rabbit Monoclonal Antibody (SPTBN2/2887R).

6712-RBM6-P1ABX

Western Blot Analysis of Human Kidney, Human Testis and HeLa lysates using Spectrin Beta 3 Recombinant Rabbit Monoclonal Antibody (SPTBN2/2887R).

Western Blot Analysis of Brain, Kidney and Testis tissue lysates of different species using Spectrin Beta 3 Recombinant Rabbit Monoclonal Antibody (SPTBN2/2887R).