p53 Tumor Suppressor Protein Antibody, IgG2b, Clone: [SPM590], Mouse, Monoclonal

Produktübersicht

• Artikelname: p53 Tumor Suppressor Protein Antibody, IgG2b, Clone: [SPM590], Mouse, Monoclonal
• Artikelnummer: NBT-7157-MSM2X-P1ABX
• Hersteller Artikelnummer: 7157-MSM2X-P1ABX
• Alternativnummer: NBT-7157-MSM2X-P1ABX-100
• Hersteller: NeoBiotechnologies
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: FC, IF, IHC, WB
• Spezies Reaktivität: Bovine, Human, Monkey
• Immunogen: Recombinant human wild type p53 protein expressed in E. coli.
• Alternative Synonym: Antigen NY-CO-13, BCC7, Cellular Tumor Antigen p53, LFS1, TP53, Transformation Related Protein 53 (TRP53), Tumor Protein p53, Tumor Suppressor p53

Produktbeschreibung

Recognizes a 53kDa protein, which is identified as p53 suppressor gene product. It reacts with the mutant as well as the wild form of p53. Its epitope maps within the N-terminus (aa 37-45) of p53. p53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. It binds to MDM2, SV40 T antigen and human papilloma virus E6 protein. Positive nuclear staining with p53 antibody has been reported to be a negative prognostic factor in breast carcinoma, lung carcinoma, colorectal, and urothelial carcinoma. Anti-p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma as well as to detect intratubular germ cell neoplasia. Mutations involving p53 are found in a wide variety of malignant tumors, including breast, ovarian, bladder, colon, lung, and melanoma.

Produkteigenschaften

• Klonalität: Monoclonal
• Klon-Bezeichnung: [SPM590]
• Molekulargewicht: 53kDa.
• Isotyp: IgG2b
• NCBI: 7157
• UniProt: P04637
• Formulierung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Antibody Type: Monoclonal Antibody

Anwendungsbeschreibung

• Anwendungsbeschreibung: Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Western Blot (1-2ug/ml), Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM

Bilder

Western Blot Analysis of human HeLa cell lysate using p53 Monoclonal Antibody (SPM590)

Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with p53 Monoclonal Antibody (SPM590)

Western Blot Analysis of human HeLa cell lysate using p53 Monoclonal Antibody (SPM590)