Recombinant p53 (Tumor Suppressor Protein) Antibody, Clone: [TP53/7082R], Rabbit, Monoclonal

Produktübersicht

• Artikelname: Recombinant p53 (Tumor Suppressor Protein) Antibody, Clone: [TP53/7082R], Rabbit, Monoclonal
• Artikelnummer: NBT-7157-RBM29-P1ABX
• Hersteller Artikelnummer: 7157-RBM29-P1ABX
• Alternativnummer: NBT-7157-RBM29-P1ABX-100
• Hersteller: NeoBiotechnologies
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: IHC, WB
• Spezies Reaktivität: Human
• Immunogen: Recombinant human full-length TP53 protein
• Alternative Synonym: Antigen NY-CO-13, BCC7, Cellular Tumor Antigen p53, LFS1, TP53, Transformation Related Protein 53 (TRP53)

Produktbeschreibung

The specificity of this monoclonal antibody to its intended target was validated by HuProtTM Array, containing more than 19,000, full-length human proteins. Recognizes a 53kDa protein, which is identified as p53 suppressor gene product. It reacts with the mutant as well as the wild form of p53 protein. p53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. It binds to MDM2, SV40 T antigen and human papilloma virus E6 protein. Positive nuclear staining with p53 antibody has been reported to be a negative prognostic factor in breast carcinoma, lung carcinoma, colorectal, and urothelial carcinoma. Anti-p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma as well as to detect intratubular germ cell neoplasia. Mutations involving p53 are found in a wide variety of malignant tumors, including breast, ovarian, bladder, colon, lung, and melanoma.

Produkteigenschaften

• Klonalität: Monoclonal
• Klon-Bezeichnung: [TP53/7082R]
• Molekulargewicht: 53kDa
• NCBI: 7157
• UniProt: P04637
• Formulierung: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Antibody Type: Recombinant Monoclonal Antibody

Anwendungsbeschreibung

• Anwendungsbeschreibung: Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes),Optimal dilutio

Bilder

Western Blot Analysis of A431 and Raji cell lysates using p53 Recombinant Rabbit Monoclonal Antibody (TP53/7082R).

Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with p53 Recombinant Rabbit Monoclonal Antibody (TP53/7082R). Inset: PBS instead of primary antibody, secondary only negative control.

SDS-PAGE Analysis of Purified p53 Recombinant Rabbit Monoclonal Antibody (TP53/7082R). Confirmation of Purity and Integrity of Antibody.