Recombinant fragment of human Ubiquitin protein (around aa 1-119) (exact sequence is proprietary)
Alternative Synonym:
Epididymis secretory protein Li 50, Polyubiquitin B, RPS27A, UBA52, UBA80, UBB, UBC, UBCEP1, UBCEP2
Ubiquitin is a highly conserved and plays an essential role in the ubiquitin-proteasome pathway. In ubiquitination process, it is first activated by forming a thiol-ester complex with the activation component E1, which is then transferred to ubiquitin-carrier protein E2, followed by to ubiquitin ligase E3 for final delivery to epsilon-NH2 of the target protein lysine residue. IkB, p53, cdc25A, Bcl-2 etc. are shown as targets of ubiquitin-proteasome process as part of regulation of cell cycle progression, differentiation, cell stress response, and apoptosis. Moreover, ubiquitin have been reported to bind covalently with pathological inclusions which are resistant to degradation e.g. neurofibrillary tangles/paired helical filaments in Alzheimers disease, Lewy bodies seen in Parkinsons disease, and Pick bodies found in Picks disease etc.
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Anwendungsbeschreibung:
Flow Cytometry (1-2ug/million cells), Western Blot (1-2ug/ml), Immunofluorescence (1-2ug/ml), Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA
SDS-PAGE Analy
Formalin-fixed, paraffin-embedded human Brain stained with Ubiquitin-Monospecific Mouse Monoclonal Antibody (UBB/1748).
Formalin-fixed, paraffin-embedded human Brain stained with Ubiquitin-Monospecific Mouse Monoclonal Antibody (UBB/1748).
SDS-PAGE Analysis Purified Ubiquitin-Monospecific Mouse Monoclonal Antibody (UBB/1748). Confirmation of Purity and Integrity of Antibody.
Western Blot Analysis of human Raji cell lysate using Ubiquitin-Monospecific Mouse Monoclonal Antibody (UBB/1748).
Flow Cytometric Analysis of PFA-fixed MCF-7 cells using Ubiquitin-Monospecific Mouse Monoclonal Antibody (UBB/1748) followed by goat anti-Mouse IgG-CF488 (Blue), Isotype Control (Red).
Analysis of Protein Array containing more than 19,000 full-length human proteins using
Western Blot Analysis of Human HeLa lysate using Ubiquitin Mouse Monoclonal Antibody (UBB/1748)
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
