PGP9.5 / UchL1 (pan-Neuronal Marker) Antibody, IgG1, Clone: [31A3], Mouse, Monoclonal

Produktübersicht

• Artikelname: PGP9.5 / UchL1 (pan-Neuronal Marker) Antibody, IgG1, Clone: [31A3], Mouse, Monoclonal
• Artikelnummer: NBT-7345-MSM1-P1ABX
• Hersteller Artikelnummer: 7345-MSM1-P1ABX
• Alternativnummer: NBT-7345-MSM1-P1ABX-100
• Hersteller: NeoBiotechnologies
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: FC, IF, IHC, WB
• Spezies Reaktivität: Bovine, Canine, Hamster, Human, Mouse, Rat
• Immunogen: Native UchL1 (PGP9.5) protein from brain
• Alternative Synonym: Gracile Axonal Dystrophy, Neuron Cytoplasmic Protein 9.5, Park5, Parkinson Disease 5, PGP95, Protein Gene Product 9.5, Ubiquitin Carboxyl-terminal Esterase L1, Ubiquitin Carboxyl-terminal Hydrolase Isozyme L1, Ubiquitin Thioesterase L1, Ubiquitin Thiolesterase L1

Produktbeschreibung

This MAb reacts with a protein of 20-30kDa, identified as PGP9.5, also known as ubiquitin carboxyl-terminal hydrolase-1 (UchL1). Initially, PGP9.5 expression in normal tissues was reported in neurons and neuroendocrine cells but later it was found in distal renal tubular epithelium, spermatogonia, Leydig cells, oocytes, melanocytes, prostatic secretory epithelium, ejaculatory duct cells, epididymis, mammary epithelial cells, Merkel cells, and dermal fibroblasts. Furthermore, immunostaining for PGP9.5 has been shown in a wide variety of mesenchymal neoplasms as well. A mutation in PGP9.5 gene is believed to cause a form of Parkinsons disease.

Produkteigenschaften

• Klonalität: Monoclonal
• Klon-Bezeichnung: [31A3]
• Molekulargewicht: 20-30kDa
• Isotyp: IgG1
• NCBI: 7345
• UniProt: P09936
• Formulierung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Antibody Type: Monoclonal Antibody

Anwendungsbeschreibung

• Anwendungsbeschreibung: Flow Cytometry (1-2ug/million cells),Immunofluorescence (1-2ug/ml), Western Blot (1-2ug/ml),Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM ED

Bilder

Western Blot A

Formalin-fixed, paraffin-embedded human brain stained with Pgp9.5 / UchL1 Mouse Monoclonal Antibody (31A3).

Western blot of UchL1 (PGP9.5) in 1) human, 2) Mouse and 3) rat brain lysate using Pgp9.5 / UchL1 Mouse Monoclonal Antibody (31A3).

Western Blot Analysis of human brain tissue lysate using Pgp9.5 / UchL1 Mouse Monoclonal Antibody (31A3).

Flow Cytometric Analysis of T98G cells using Pgp9.5 / UchL1 Mouse Monoclonal Antibody (31A3) followed by Goat anti-Mouse IgG-CF488 (Blue), Isotype Control (Red).

Immunofluorescence Analysis of T98G cells labeling Pgp9.5 with Pgp9.5 / UchL1 Mouse Monoclonal Antibody (31A3) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Nucspot (Red)

Western Blot Analysis of Human Brain, Mouse Brain, Rat Brain, Guinea pig Brain and Hamster Brain tissue lysates using PGP9.5 / UchL1 Mouse Monoclonal Antibody (31A3).

SDS-PAGE Analysis of Purified UCHL-1 Mouse Monoclonal Antibody(31A3). Confirmation of Purity and Integrity of Antibody.

Western Blot Analysis of human brain tissue lysate using PGP9.5 / UchL1 Mouse Monoclonal Antibody (31A3).