Villin (GI-Mucosal & Urogenital Brush Border Marker) Antibody, IgG1, Clone: [VIL1/2376], Mouse, Monoclonal

Artikelnummer: NBT-7429-MSM6-P0
Artikelname: Villin (GI-Mucosal & Urogenital Brush Border Marker) Antibody, IgG1, Clone: [VIL1/2376], Mouse, Monoclonal
Artikelnummer: NBT-7429-MSM6-P0
Hersteller Artikelnummer: 7429-MSM6-P0
Alternativnummer: NBT-7429-MSM6-P0-20,NBT-7429-MSM6-P0-100
Hersteller: NeoBiotechnologies
Wirt: Mouse
Kategorie: Antikörper
Applikation: IHC
Spezies Reaktivität: Human
Immunogen: A recombinant fragment (around aa 179-311) of human Villin protein (exact sequence is proprietary)
Alternative Synonym: VIL1, Villin-1, Villin1
Recognizes a protein of 95kDa, which is identified as villin. It is a major constituent in the microvilli, which compose the brush border of epithelial cells forming absorptive surfaces of the intestinal and renal proximal tubular epithelia. Anti-Villin labels the brush border area in the gastrointestinal mucosal epithelium and urogenital tract. Among neoplasms, villin is predominantly expressed in tumors of colorectal origin. Antibody to villin is useful in identifying malignant cells from primary and metastatic colorectal carcinomas. This antibody also labels Merkel cells of the skin.
Klonalität: Monoclonal
Klon-Bezeichnung: [VIL1/2376]
Molekulargewicht: 93kDa
Isotyp: IgG1
NCBI: 7429
UniProt: P09327
Formulierung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type: Monoclonal Antibody
Anwendungsbeschreibung: ELISA (For coating, order antibody without BSA), ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followe
SDS-PAGE Analysis Purified Villin Mouse Monoclonal Antibody (VIL1/2376).
Formalin-fixed, paraffin-embedded human colon carcinoma stained with Villin Mouse Monoclonal Antibody (VIL1/2376). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.
SDS-PAGE Analysis Purified Villin Mouse Monoclonal Antibody (VIL1/2376). Confirmation of Integrity and Purity of Antibody.