Recognizes a protein of 150kDa, which is identified as the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120 150kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70 80kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This antibody recognizes only the 150kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.
200ug/ml of Ab purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Anwendungsbeschreibung:
Western Blot (1-2ug/ml), Flow Cytometry (0.5-1ug/million cells), Immunofluorescence (1-2ug/ml), ,Immunohistology (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 7.5-8.5
Formalin-fixed, paraffin-embedded human Uterus stained with Caldesmon Rabbit Polyclonal Antibody.
Formalin-fixed, paraffin-embedded Rat Uterus stained with Caldesmon Rabbit Polyclonal Antibody.
Western Blot Analysis of human ovary tissue lysate using Cal
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