Calcium dependent protease small subunit 1, Calcium-activated neutral proteinase 1, Calpain 1 large subunit, Calpain mu-type, Calpain regulatory subunit, Calpain small subunit 1, Calpain, large polypeptide L1, Calpain-1 catalytic subunit, CANP small subunit, CANP1, CANPL1, CAPN1, Cell proliferation-inducing gene 30 protein, Micromolar-calpain, Mu Calpain
Calpain 1 is an intracellular calcium-dependent protease that cleaves cytoskeletal and sub-membranous proteins. Calpains are non-lysosomal, calcium-activated intracellular cysteine proteases. Calpains mediate specific Ca2+- dependent processes including cell fusion, mitosis and meiosis. Calpains are heterodimers of a small regulatory subunit and one of three large catalytic subunits, designated Calpain 1, Calpain 2 and Calpain p94. Calpastatin regulates Calpain by inhibiting both the proteolytic activity of Calpain and its binding to membranes. Calpastatin exists in two types, tissue type and erythrocyte type, resulting from both alternative splicing and proteolytic processing. Calpain 1 co-localizes with human leukocyte antigen-DR (HLA-DR) on activated microglia in the aging brain. Calpain influences the process of spermatogenesis and the events preceding fertilization, such as the acrosome reaction.
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Anwendungsbeschreibung:
ELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA), Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95
SDS-PAGE Analys
Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Calpain 1 Mouse Monoclonal Antibody (CAPN1/1530).
Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with Calpain 1 Mouse Monoclonal Antibody (CAPN1/1530).
SDS-PAGE Analysis of Purified Calpain 1 Mouse Monoclonal Antibody (CAPN1/1530). Confirmation of Purity and Integrity of Antibody.
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