Recombinant Cyclin E (G1/S-Phase Cyclin) Antibody, Clone: [CCNE1/4935R], Rabbit, Monoclonal
Artikelnummer:
NBT-898-RBM5-P0
Hersteller Artikelnummer:
898-RBM5-P0
Alternativnummer:
NBT-898-RBM5-P0-20,NBT-898-RBM5-P0-100
Hersteller:
NeoBiotechnologies
Wirt:
Rabbit
Kategorie:
Antikörper
Applikation:
IHC, WB
Spezies Reaktivität:
Human
Immunogen:
Recombinant human Cyclin E (CCNE1) protein fragment (around aa 10-176) (exact sequence is proprietary)
Alternative Synonym:
CCNE1, Cyclin E1, G1/S-specific cyclin-E1
Cyclin E belongs to the highly conserved cyclin family, whose members exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclins function as regulators of CDK kinases. Cyclin E forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. Cyclin E accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Cyclin E overexpression has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis.
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Recombinant Monoclonal Antibody
Anwendungsbeschreibung:
Western Blot (1-2ug/ml), ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for
Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with Cyclin E Recombinant Rabbit Monoclonal Antibody (CCNE1/4935R).
Formalin-fixed, paraffin-embedded human endometrium stained with Cyclin E Recombinant Rabbit Monoclonal Antibody (CCNE1/4935R).
Western Blot Analysis of HCT-116 cell lysate using Cyclin E Recombinant Rabbit Monoclonal Antibody (CCNE1/4935R).
SDS-PAGE Analysis of Purified Cyclin E Recombinant Rabbit Monoclonal Antibody (CCNE1/4935R). Confirmation of Purity and Integrity of Antibody.
Western Blot Analysis of HCT-116, HeLa, Jurkat and MCF7 cell lysates using Cyclin E Recombinant Rabbit Monoclonal Antibody (CCNE1/4935R).
Western Blot Analysis of HCT-116 cell lysate using Cyclin E Recombinant Rabbit Monoclonal Antibody (CCNE1/4935R).
Western Blot Analysis of HCT-116, MCF7 and HeLa cell lysates using Cyclin E Recombinant Rabbit Monoclonal Antibody (CCNE1/4935R).
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
