APY, ATOPY, B-lymphocyte cell-surface antigen B1, Bp35, Fc epsilon receptor I beta chain, Fc Fragment of IgE high affinity I receptor for beta polypeptide, FCER1B, High affinity immunoglobulin epsilon receptor subunit beta, IgE Fc receptor subunit beta, IGEL, IGER, IGHER, Leukocyte surface antigen Leu-16, Ly44, MS4A1, MS4A2
Recognizes a protein of 30-33kDa, which is identified as CD20. Its epitope is located in the cytoplasmic domain of CD20 and was, therefore, ascribed as CD20cy in the 5th Workshop. CD20 is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. This MAb can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood and B cell localization in tissues. It reacts with the majority of B-cells present in peripheral blood and lymphoid tissues and their derived lymphomas. In lymphoid tissue, germinal center blasts and B-immunoblasts are particularly reactive. It is a reliable antibody for ascribing a B-cell phenotype in known lymphoid tissues. Rarely, CD20-positive T-cell lymphomas have been reported. Reactivity has also been noted with Reed-Sternberg cells in cases of Hodgkin s disease, particularly of lymphocyte predominant type.
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Anwendungsbeschreibung:
Flow Cytometry (0.5-1ug/million cells), Immunofluorescence (1-2ug/ml), Western Blotting (1-2ug/ml),,Immunohistology (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate bu
Formalin-fixed, paraffin-embedded human Tonsil stained with CD20 Mouse Monoclonal Antibody (L26).
Formalin-fixed, paraffin-embedded human Lymphoma stained with CD20 Mouse Monoclonal Antibody (L26).
Flow Cytometric Analysis of Raji Cells. using CD20 Mouse
Formalin-fixed, paraffin-embedded human Tonsil stained with CD20 Mouse Monoclonal Antibody (L26).
Formalin-fixed, paraffin-embedded human Lymphoma stained with CD20 Mouse Monoclonal Antibody (L26).
Flow Cytometric Analysis of Raji Cells. using CD20 Mouse MAb (L26) followed by Goat anti-mouse IgG-CF488 (Blue), Isotype Control (Red).
Formalin-fixed, paraffin-embedded human Lymphoma stained with CD20 Mouse Monoclonal Antibody (L26).
SDS-PAGE Analysis Purified CD20 Mouse Monoclonal Antibody (L26). Confirmation of Integrity and Purity of Antibody.
Immunofluorescence staining of Raji cells using CD20 Mouse Monoclonal Antibody (L26), followed by goat anti-mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot.
Western Blot Analysis of Raji cell lysate using CD20 Mouse Monoclonal Antibody (L26).
Western Blot Analysis of Raji cell lysate using CD20 Mouse Monoclonal Antibody (L26).
Western Blot Analysis of Raji cell lysate using CD20 Mouse Monoclonal Antibody (L26).
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