Recombinant CD20 / MS4A1 (B-Cell Marker) Antibody, Clone: [MS4A1/7015R], Rabbit, Monoclonal

Artikelname:	Recombinant CD20 / MS4A1 (B-Cell Marker) Antibody, Clone: [MS4A1/7015R], Rabbit, Monoclonal
Artikelnummer:	NBT-931-RBM19-P1ABX
Hersteller Artikelnummer:	931-RBM19-P1ABX
Alternativnummer:	NBT-931-RBM19-P1ABX-100
Hersteller:	NeoBiotechnologies
Wirt:	Rabbit
Kategorie:	Antikörper
Applikation:	IHC, WB
Spezies Reaktivität:	Canine, Guinea pig, Hamster, Human, Mouse, Rabbit, Rat
Immunogen:	Recombinant fragment (around aa 97-297) corresponding to C-terminal of human CD20 protein (exact sequence is proprietary)
Alternative Synonym:	APY, ATOPY, B-lymphocyte cell-surface antigen B1, Bp35, Fc epsilon receptor I beta chain, Fc Fragment of IgE high affinity I receptor for beta polypeptide, FCER1B, High affinity immunoglobulin epsilon receptor subunit beta, IgE Fc receptor subunit beta, IGEL, IGER, IGHER, LEU16, Leukocyte surface antigen Leu-16, Ly44, MS4A1, MS4A2

Recognizes a protein of 30-33kDa, which is identified as CD20. It is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. This MAb can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood and B cell localization in tissues. It reacts with the majority of B-cells present in peripheral blood and lymphoid tissues and their derived lymphomas. In lymphoid tissue, germinal center blasts and B-immunoblasts are particularly reactive. It is a reliable antibody for ascribing a B-cell phenotype in known lymphoid tissues. Rarely, CD20-positive T-cell lymphomas have been reported. Reactivity has also been noted with Reed-Sternberg cells in cases of Hodgkin s disease, particularly of lymphocyte predominant type.

Klonalität:	Monoclonal
Klon-Bezeichnung:	[MS4A1/7015R]
Molekulargewicht:	33-37kDa
NCBI:	931
UniProt:	P11836
Formulierung:	200ug/ml of Ab purified by Protein A Column. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:	Recombinant Monoclonal Antibody

Anwendungsbeschreibung:

Western Blot (1-2ug/ml),,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 &degC followed by cooling at RT for 2

	Formalin-fi
	SDS-PAGE Analysis Purified CD20 Recombinant Rabbit Monoclonal Antibody (MS4A1/7015R). Confirmation of Purity and Integrity of Antibody.
	Western blot analysis of Raji cell lysate using CD20 Recombinant Rabbit Monoclonal Antibody (MS4A1/7015R).
	Formalin-fixed, paraffin-embedded human tonsil stained with CD20 Recombinant Rabbit Monoclonal Antibody (MS4A1/7015R). HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.
	IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using MS4A1/7015R at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.
	Formalin-fixed, paraffin-embedded human tonsil stained with CD20 Recombinant Rabbit Monoclonal Antibody (MS4A1/7015R). Inset: PBS instead of primary antibody, secondary only negative control.
	IHC analysis of formalin-fixed, paraffin-embedded human heart. Negative tissue control using MS4A1/7015R at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.
	Western blot analysis of Human Liver and Hep G2 lysates using Glypican-3 / GPC3 Mouse Monoclonal Antibody (GPC3/12697).
	Western blot analysis of human liver tissue and HepG2 cell lysates using Glypican-3 Mouse Monoclonal Antibody (GPC3/12698).
	Western blot analyses of human lung, mouse lung, rat lung, hamster lung, guinea pig lung and rabbit lung tissue lysates using MUC1 Mouse Monoclonal Antibody (MUC1/845).
	Western blot analyses of human lung, mouse lung, rat lung, hamster lung, guinea pig lung and rabbit lung tissue lysates using MUC-1 Recombinant Rabbit Monoclonal Antibody (MUC1/2818R).
	Western blot analysis of Human Intestine, Mouse Intestine, Rat Intestine, Hamster Intestine, Guinea pig Intestine and Rabbit Intestine tissue lysates using CD20 Recombinant Rabbit Monoclonal Antibody (MS4A1/7015R).