QKI / Protein Quaking Antibody, IgG2c, Clone: [PCRP-QKI-2F10], Mouse, Monoclonal

Produktübersicht

• Artikelname: QKI / Protein Quaking Antibody, IgG2c, Clone: [PCRP-QKI-2F10], Mouse, Monoclonal
• Artikelnummer: NBT-9444-MSM1-P0
• Hersteller Artikelnummer: 9444-MSM1-P0
• Alternativnummer: NBT-9444-MSM1-P0-20,NBT-9444-MSM1-P0-100
• Hersteller: NeoBiotechnologies
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: ELISA, FC, IF, WB
• Spezies Reaktivität: Human
• Immunogen: Recombinant full-length human QKI protein
• Alternative Synonym: Homolog of mouse quaking QKI KH domain RNA binding protein, HQK1, Protein quaking, QK1, QK3, QKI, QKI1, Quaking homolog KH domain RNA binding mouse, Quaking isoform 1, Quaking protein, RNA binding protein HQK

Produktbeschreibung

QKI, also known as HKQ, QK, QK3 or quaking, is a 341 amino acid protein that localizes to both the cytoplasm and the nucleus and contains one KH domain. Expressed in the frontal cortex of the brain, QKI functions as an RNA-binding protein that plays an important role in myelinization and specifically binds to the RNA core sequence 5-NACUAAY-N(1,20)-UAAY-3. Additionally, QKI regulates pre-mRNA splicing, and mRNA export and is involved in protecting and promoting the stability of select mRNAs. QKI may be methylated by PRMT1 and may also be phosphorylated at its C-terminus, an event that decreases QKI mRNA-binding affinity. Defects or deletions in the gene encoding QKI are associated with astrocytic tumors and may be involved in the pathogenesis of schizophrenia. Multiple isoforms of QKI exist due to alternative splicing events.

Produkteigenschaften

• Klonalität: Monoclonal
• Klon-Bezeichnung: [PCRP-QKI-2F10]
• Molekulargewicht: 37.6kDa
• Isotyp: IgG2c
• NCBI: 9444
• UniProt: Q96PU8
• Formulierung: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Antibody Type: Monoclonal Antibody

Anwendungsbeschreibung

• Anwendungsbeschreibung: ELISA (concentration assay dependent), Flow Cytometry (1-2ug/million cells),,Immunoprecipitation (1-2ug per 100-500ug of total protein (1ml of cell lysate)), ,Immunofluorescence (1-2ug/ml), Western Blot (1-2ug/ml), ,Optimal dilution for a specific applic

Bilder

SDS-PAGE Analysis Purified QKI Mouse Monoclonal Antibody (PCRP-QKI-2F10) Confirmation of Purity and Integrity of Antibody.

Flow cytometric analysis of PFA-fixed HeLa cells. QKI Mouse Monoclonal Antibody (PCRP-QKI-2F10) followed by goat anti-mouse IgG-CF488 (blue), isotype control (red).

Flow cytometric analysis of PFA-fixed K562 cells. QKI Mouse Monoclonal Antibody (PCRP-QKI-2F10) followed by goat anti-mouse IgG-CF488 (blue), isotype control (red).

Flow cytometric analysis of PFA-fixed U87 cells. QKI Mouse Monoclonal Antibody (PCRP-QKI-2F10) followed by goat anti-mouse IgG-CF488 (blue), isotype control (red).

Flow cytometric analysis of PFA-fixed MCF7 cells. QKI Mouse Monoclonal Antibody (PCRP-QKI-2F10) followed by goat anti-mouse IgG-CF488 (blue), isotype control (red).

Immunofluorescence Analysis of PFA-fixed U87 cells. Staining localized to the nucleoplasm using QKI Mouse Monoclonal Antibody (PCRP-QKI-2F10) followed by goat anti-mouse IgG-CF488 (green). CF640R phalloidin (red).

Immunofluorescence Analysis of K562 cells stained using QKI Mouse Monoclonal Antibody (PCRP-QKI-2F10) followed by goat anti-mouse IgG-CF488 (green). CF640R phalloidin (red).

Immunofluorescence Analysis of PFA-fixed MCF7 cells stained using QKI Mouse Monoclonal Antibody (PCRP-QKI-2F10) followed by goat anti-mouse IgG-CF488 (green). CF640R phalloidin (red).