This MAb recognizes protein of 26kDa-60kDa, which is identified as CD63. Its epitope is different from that of MAb LAMP3/529. The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression.
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Flow Cytometric Anal
Flow cytometric analysis of human CD63 on MCF-7 cells. Black: cells alone, Green: isotype control, Red: PE-labeled CD63 Mouse Monoclonal Antibody (NKI/C3).
Immunofluorescent Analysis of HeLa cells using CF488-labeled CD63 Mouse Monoclonal Antibody (NKI/C3). F-actin filaments are labeled with Dylight 554 phalloidin (red). DAPI was used to stain the cell nucleus (blue).
Formalin-fixed, paraffin-embedded human melanoma stained with CD63 Mouse Monoclonal Antibody (NKI/C3) (AEC Chromogen)
Flow Cytometric Analysis of PFA-fixed U87MG cells. CD63 Mouse Monoclonal Antibody (NKI/C3) followed by goat anti-mouse IgG-CF488 (blue), isotype control (red).
SDS-PAGE Analysis Purified CD63 Mouse Monoclonal Antibody (NKI/C3). Confirmation of Purity and Integrity of Antibody.
Flow cytometric analysis of human CD63 on MCF-7 cells. Black: cells alone, Green: isotype control, Red: PE-labeled CD63 Mouse Monoclonal Antibody (NKI/C3).
Flow cytometry analysis of live MCF-7 cells. Unstained (gray) or stained with biotin-labeled CD63 monoclonal antibody (NKI-C3) followed by streptavidin-CF568 (orange).
Flow cytometric analysis of human CD63 on MCF-7 cells. Black: cells alone, Green: isotype control, Red: PE-labeled CD63 Mouse Monoclonal Antibody (NKI/C3).
Flow Cytometric Analysis of PFA-fixed U87MG cells. CD63 Mouse Monoclonal Antibody (NKI/C3) followed by goat anti-mouse IgG-CF488 (blue), isotype control (red).
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