CLIP, DHLAG, Gamma chain of class II antigens, HLA class II histocompatibility antigen gamma chain, HLA-DR antigens-associated invariant chain, HLADR-gamma (HLADG), Ia antigen-associated invariant chain, la-gamma, Major histocompatibility complex class II invariant chain, MHC HLA-DR gamma chain
It recognizes a protein of ~35kDa, identified as CD74 (Workshop IV). CD74 is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. CD74 is expressed primarily by antigen presenting cells, such as B-lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages, and monocytes, and many epithelial cells. Anti-CD74 stains predominantly germinal center lymphocytes and B-cell lymphomas, but rarely T-cell lymphomas. Anti-CD74 has been shown to be useful in differentiating atypical fibroxanthoma (-) from malignant fibrous histiocytoma (+).
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Recombinant Monoclonal Antibody
Anwendungsbeschreibung:
Flow Cytometry (1-2ug/million cells), Western Blot (1-2ug/ml),Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C
Formalin-fixed, paraffin-embedded human Tonsil stained with CD74 Recombinant Rabbit Monoclonal Antibody (CLIP/3127R).
SDS-PAGE Analysis of Purified CD74 Recombinant Rabbit Monoclonal Antibody (CLIP/3127R). Confirmation of Purity and Integrity of Antibody.
Western Blot Analysis of Human 293T cell lysate using CD74 Recombinant Rabbit Monoclonal Antibody (CLIP/3127R).
Flow Cytometric Analysis of Raji cells. CD74 Recombinant Rabbit Monoclonal Antibody (CLIP/3127R) followed by goat anti-Mouse IgG-CF488 (Blue). Isotype Control (Red).
Western Blot Analysis of Raji lysate using CD74 Rabbit Recombinant Monoclonal Antibody (CLIP/3127R).
Western Blot Analysis of Human Spleen and Raji lysates using CD74 Recombinant Rabbit Monoclonal Antibody (CLIP/3127R).
Western Blot Analysis of Human Spleen, Mouse Spleen, Rat Spleen, Hamster Spleen, Guinea pig Spleen and Rabbit Spleen tissue lysates using CD74 Recombinant Rabbit Monoclonal Antibody (CLIP/3127R).
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
