E-Cadherin (CDH1) / CD324 (Intercellular Junction Marker) Antibody, IgG1, Clone: [4A2], Mouse, Monoclonal

Produktübersicht

• Artikelname: E-Cadherin (CDH1) / CD324 (Intercellular Junction Marker) Antibody, IgG1, Clone: [4A2], Mouse, Monoclonal
• Artikelnummer: NBT-999-MSM4-P1
• Hersteller Artikelnummer: 999-MSM4-P1
• Alternativnummer: NBT-999-MSM4-P1-100
• Hersteller: NeoBiotechnologies
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: FC, IF, IHC, WB
• Spezies Reaktivität: Human, Mouse
• Immunogen: Recombinant human E-Cadherin protein
• Alternative Synonym: Arc 1, cadherin 1 type 1 E-cadherin, Cadherin1, CAM 120/80, CD324, CDHE, E-Cad/CTF3, ECAD, Epithelial cadherin, epithelial calcium dependent adhesion protein, Liver cell adhesion molecule (LCAM), Uvomorulin (UVO)

Produktbeschreibung

Recognizes a protein of 120-80kDa, identified as E-cadherin. Cadherins comprise a family of Ca2+-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. The classical cadherins, E-, N- and P-cadherin, consist of large extracellular domains characterized by a series of five homologous NH2 terminal repeats. The relatively short intracellular domains interact with a variety of cytoplasmic proteins, such as A°a-catenin, to regulate cadherin function. E-cadherin plays an important role in epithelial cell adhesion. A decreased expression of E-cadherin is associated with metastatic potential and poor prognosis in breast cancer, prostate and esophageal cancer. In combination with p120 Catenin, it is useful for the differentiation between ductal (E-cadherin +) and lobular (E-cadherin -) breast carcinomas. It may also help in diagnosis of mesothelioma.

Produkteigenschaften

• Klonalität: Monoclonal
• Klon-Bezeichnung: [4A2]
• Molekulargewicht: 120-80kDa (Mature), 135kDa (Precursor)
• Isotyp: IgG1
• NCBI: 999
• UniProt: P12830
• Formulierung: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Bilder

Western Blot Analysis of MCF-7 cell lysate using E-Cadherin Mouse Monoclonal Antibody (4A2).

Immunofluorescence analysis of MeOH-fixed MCF-7 cells. E-Ca

Formalin-fixed, paraffin-embedded human skin stained with E-Cadherin Mouse Monoclonal Antibody (4A2).

Western Blot Analysis of MCF-7 cell lysate using E-Cadherin Mouse Monoclonal Antibody (4A2).

Immunofluorescence analysis of MeOH-fixed MCF-7 cells. E-Cadherin Mouse Monoclonal Antibody (4A2) followed by goat anti-mouse IgG-CF488 (green). Nuclei counterstained with RedDot.

Flow cytometric analysis of trypsinized MCF-7 cells. E-Cadherin Mouse Monoclonal Antibody (4A2) followed by goat anti-mouse IgG-CF488 (blue), isotype control (red).

SDS-PAGE Analysis Purified E-Cadherin Mouse Monoclonal Antibody (4A2) Confirmation of Integrity and Purity of Antibody.

Western Blot Analysis of human placenta tissue lysate using CDH1 Mouse Monoclonal Antibody (4A2).