Recognizes a protein of 120-80kDa, identified as E-cadherin. Cadherins comprise a family of Ca2+-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. The classical cadherins, E-, N- and P-cadherin, consist of large extracellular domains characterized by a series of five homologous NH2 terminal repeats. The relatively short intracellular domains interact with a variety of cytoplasmic proteins, such as A°a-catenin, to regulate cadherin function. E-cadherin plays an important role in epithelial cell adhesion. A decreased expression of E-cadherin is associated with metastatic potential and poor prognosis in breast cancer, prostate and esophageal cancer. In combination with p120 Catenin, it is useful for the differentiation between ductal (E-cadherin +) and lobular (E-cadherin -) breast carcinomas. It may also help in diagnosis of mesothelioma.
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Recombinant Monoclonal Antibody
Anwendungsbeschreibung:
Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Western Blot (1-2ug/ml), ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1m
SDS-PAGE A
Formalin-fixed, paraffin-embedded human colon carcinoma stained with E-Cadherin Mouse Recombinant Monoclonal Antibody (rCDH1/1525).
Western Blot Analysis of MCF-7 cell lysate using E-Cadherin Mouse Recombinant Monoclonal Antibody (rCDH1/1525).
SDS-PAGE Analysis Purified E-Cadherin Mouse Recombinant Monoclonal (rCDH1/1525). Confirmation of Purity and Integrity of Antibody.
Immunofluorescence Analysis of MCF-7 cells labeling CHD1 with E-Cadherin Mouse Recombinant Monoclonal Antibody (rCDH1/1525) followed by goat anti-mouse IgG-CF488 (green). Nuclear counterstain is RedDot.
Analysis of Protein Array containing >19,000 full-length human proteins using E-Cadherin Recombinant Mouse Monoclonal Antibody (rCDH1/1525) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD&apos,s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD&apos,s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Western Blot Analysis of human skin tissue lysate using CDH1 Recombinant Mouse Monoclonal Antibody (rCDH1/1525).
Western Blot Analysis of human placenta tissue lysate using CDH1 Recombinant Mouse Monoclonal Antibody (rCDH1/1525).
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
