Recombinant E-Cadherin (CDH1) / CD324 (Intercellular Junction Marker) Antibody, Clone: [CDH1/2208R], Rabbit, Monoclonal

Produktübersicht

• Artikelname: Recombinant E-Cadherin (CDH1) / CD324 (Intercellular Junction Marker) Antibody, Clone: [CDH1/2208R], Rabbit, Monoclonal
• Artikelnummer: NBT-999-RBM8-P0
• Hersteller Artikelnummer: 999-RBM8-P0
• Alternativnummer: NBT-999-RBM8-P0-20,NBT-999-RBM8-P0-100
• Hersteller: NeoBiotechnologies
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: FC, IF, IHC, WB
• Spezies Reaktivität: Human, Mouse
• Immunogen: Recombinant fragment (around aa600-800) of human E-Cadherin protein (exact sequence is proprietary)
• Alternative Synonym: Arc 1, cadherin 1 type 1 E-cadherin, Cadherin1, CAM 120/80, CD324, CDH1, CDHE, E-Cad/CTF3, ECAD, Epithelial cadherin, epithelial calcium dependent adhesion protein, Liver cell adhesion molecule (LCAM), Uvomorulin (UVO)

Produktbeschreibung

Recognizes a protein of 120-80kDa, identified as E-cadherin. Cadherins comprise a family of Ca2+-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. The classical cadherins, E-, N- andP-cadherin, consist of large extracellular domains characterized by a series of five homologous NH2 terminal repeats. The relatively short intracellular domains interact with a variety of cytoplasmic proteins, such as A°a-catenin, to regulate cadherin function.E-cadherin plays an important role in epithelial cell adhesion. A decreased expression of E-cadherin is associated with metastatic potential and poor prognosis in breast cancer, prostate and esophageal cancer. In combination with p120 Catenin, it is useful for the differentiation between ductal (E-cadherin +) and lobular (E-cadherin -) breast carcinomas. It may also help in diagnosis of mesothelioma.

Produkteigenschaften

• Klonalität: Monoclonal
• Klon-Bezeichnung: [CDH1/2208R]
• Molekulargewicht: 120-80kDa (Mature), 135kDa (Precursor)
• NCBI: 999
• UniProt: P12830
• Formulierung: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Antibody Type: Recombinant Monoclonal Antibody

Anwendungsbeschreibung

• Anwendungsbeschreibung: Flow Cytometry (1-2ug/million cells),Immunofluorescence (1-2ug/ml), Western Blot(1-2ug/ml), ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM

Bilder

SDS-PAGE Analysis Pu

Formalin-fixed, paraffin-embedded human cervix stained with E-CadherinRabbit RecombinantMonoclonal Antibody (CDH1/2208R).

Western Blot Analysis of MCF-7 cell lysate using E-Cadherin Rabbit RecombinantMonoclonal Antibody (CDH1/2208R).

SDS-PAGE Analysis Purified E-Cadherin Rabbit RecombinantMonoclonal (CDH1/2208R). Confirmation of Purity and Integrity of Antibody.

Immunofluorescence Analysis of MCF-7cells labeling E-Cadherin with E-Cadherin Rabbit RecombinantMonoclonal Antibody (CDH1/2208R) followed bygoat anti-mouse IgG-CF488. The nuclear counterstain is RedDot.

Flow Cytometric Analysis of MCF-7cells using E-Cadherin Rabbit RecombinantMonoclonal Antibody (CDH1/2208R) followed by goat anti-mouse IgG-CF488 (blue), isotype control (red).

Western Blot Analysis of human placenta tissue lysate using CDH1 Recombinant Rabbit Monoclonal Antibody (CDH1/2208R).

Western Blot Analysis of Human Skin tissue lysate using E-Cadherin Recombinant Rabbit Monoclonal Antibody (CDH1/2208R).

Western Blot Analysis of human placenta tissue lysate using E-Cadherin Recombinant Rabbit Monoclonal Antibody (CDH1/2208R).