Reacts with a common epitope of human major histocompatibility (MHC) class II antigens, HLA-DP, -DQ and -DR. Human MHC class II antigens are transmembrane glycoproteins composed of an alpha chain (36kDa) and a beta chain (27kDa). They are expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, and thymic epithelial cells and are also present on activated T lymphocytes. Human MHC class II genes are located in the HLA-D region that encodes at least six alpha and ten beta chain genes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T-helper cells. They, therefore, have a critical role in the initiation of the immune response.
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Recombinant Monoclonal Antibody
Anwendungsbeschreibung:
Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Western Blot (1-2ug/ml), ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1m
Flow Cyt
Formalin-fixed, paraffin-embedded human tonsil stained with HLA-Pan Mouse Recombinant Monoclonal Antibody (rHLA-Pan/3475).
Formalin-fixed, paraffin-embedded human tonsil stained with HLA-Pan Mouse Recombinant Monoclonal Antibody (rHLA-Pan/3475).
Flow Cytometric Analysis of human Raji cells. HLA-Pan Mouse Recombinant Monoclonal Antibody (rHLA-Pan/3475) followed by goat anti-mouse IgG-CF488 (blue), isotype control (red).
Immunofluorescence Analysis of human Raji cells. HLA-Pan Mouse Recombinant Monoclonal Antibody (rHLA-Pan/3475) followed by goat anti-mouse IgG-CF488 (green). Nuclear counterstain is RedDot.
Western Blot Analysis of (1) Ramos cell and (2) human spleen tissue lysate using HLA-Pan Mouse Recombinant Monoclonal Antibody (rHLA-Pan/3475).
SDS-PAGE Analysis Purified HLA-Pan Mouse Recombinant Monoclonal (rHLA-Pan/3475). Confirmation of Purity and Integrity of Antibody.
Western Blot Analysis of Daudi cell lysate using Pan-HLA-II (DP, DQ, DR) Recombinant Mouse Monoclonal Antibody (rHLA-Pan/3475).
Western blot analysis of Human Intestine, Mouse Intestine, Rat Intestine, Hamster Intestine, Guinea pig Intestine, Human Lung, Mouse Lung, Rat Lung, Hamster Lung and Guinea pig Lung tissue lysates using using HLA-Pan Recombinant Rabbit Monoclonal Antibody (rHLA-Pan/3475).
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
