It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
Klonalität:
Monoclonal
Klon-Bezeichnung:
[BTN/403]
Molekulargewicht:
244Da
Isotyp:
IgG1
NCBI:
Not Applicable
Formulierung:
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Anwendungsbeschreibung:
Confocal Microscopy, In situ Nucleic Acid Hybridization, Flow Cytometry (0.5-1ug/million cells), ,Immunofluorescence (0.5-1ug/ml), Western Blot (0.5-2ug/ml), ,Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT),(Staining of formalin-f
Formalin-fixed, paraffin-embedded human kidney. Endogenous biotin stained with Biotin Mouse Monoclonal Antibody (BTN/403).
SDS-PAGE Analysis of Purified BTN Mouse Monoclonal Antibody (BTN/403). Confirmation of Purity and Integrity of Antibody.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
